C5a (His-Tag), murine recombinant protein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Mouse complement C5a is a 77-amino acid peptide and is generated during complement activation from the α-chain of complement C5. Its molecular mass is 9 kDa. Mouse C5a shares 60% and 82% amino acid sequence identity to human C5a and rat C5a, respectively. Mouse C5a binds to a signaling G-protein coupled receptor (GPCR). It also triggers an oxidative burst in neutrophils and macrophages, and induces release of histamine in mast cells and basophils. C5a is necessary to the early priming stages of hepatocyte regeneration. Interception of C5a receptor signaling resulted in inhibiting of IL-6/TNFα induction and lack of C5a and C3 receptor stimulation attenuated nuclear factor–κB/STAT-3 activation after hepatectomy. The 9 kDa mouse C5a protein expressed in E. coli has a N-terminal his-tag sequence.
| Gene ID: | 15139 | Accession #: | NP_034536 |
| Alternate Names: | Recombinant murine C5a (His-Tag), murine C5a, m-C5a, rm-C5a, recombinant murine C5a, murine C5a (His-tag), complement C5a, Complement Component C5a, Complement component 5a, complement fragment C5a, Complement Fragments C5a, complement-derived C5a, Complement C5a Protein | ||
| Source: | Escherichia Coli. | M.Wt: | 9 kDa |
| Appearance: | Lyophilized C5a protein | Storage: | Store at -20°C |
| Formulation: | Sterile filtered and lyophilized mouse c5a protein with no additives. | ||
| Solubility: | Mouse C5a protein can be reconstituted to a concentration of 1.0 mg/ml in ice-cold water containing 2.5 mg/ml BSA. The C5a solution should be aliquoted and immediately stored at -70°C. | ||
| Shipping Condition: | Blue ice. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||
| Determined by enzyme release assay of myeloperoxidase. | ||||||
| ED50 | = 1-3 nM. | |||||