AMI5/Eosin Y disodium salt
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
作用于Hmt1p和PRMT1,IC50分别为0.78和1.41 μM。
AMI5/Eosin Y disodium salt是一种非选择性蛋白甲基转移酶抑制剂。
赖氨酸和精氨酸残基的翻译后蛋白甲基化与基因表达调节有关。蛋白甲基转移酶的酶活性用于共价修饰,以控制基因转录。
体外:AMI5/Eosin Y disodium salt已经被鉴定为SAM结合的竞争性抑制剂,并且已证实不仅抑制PRMTs,还抑制Set7和disruptor of telomeric silencing 1-like (DOT1L) MTases的赖氨酸甲基化。AMI5/Eosin Y disodium salt及其类似物AMI-1已被用作开发新型MTase特异性抑制剂的先导化合物。此外,AMI5/Eosin Y disodium salt可以在体外抑制Set7并减少血管内皮细胞中的H3K4m1。[1]。
体内:目前尚无动物研究报告。
临床试验:到目前为止,AMI-5仍处于临床前研究阶段。
参考文献:
[1] Okabe J,Fernandez AZ,Ziemann M,Keating ST,Balcerczyk A,El-Osta A. Endothelial transcriptome in response to pharmacological methyltransferase inhibition. ChemMedChem.2014 Aug;9(8):1755-62.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 691.85 |
Cas No. | 17372-87-1 |
Formula | C20H6Br4Na2O5 |
Solubility | ≥69.2 mg/mL in DMSO; ≥27 mg/mL in EtOH; ≥48.6 mg/mL in H2O |
Chemical Name | sodium 2-(2,4,5,7-tetrabromo-6-oxido-3-oxo-3H-xanthen-9-yl)benzoate |
SDF | Download SDF |
Canonical SMILES | BrC1=C([O-])C(Br)=C2C(C(C3=CC=CC=C3C([O-])=O)=C4C=C(Br)C(C(Br)=C4O2)=O)=C1.[Na+].[Na+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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