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Catalog No. A4448

Actinomycin D

（别名：放线菌素 D）

RNA聚合酶抑制剂

规格	价格	货期	数量
25mg	￥1550.00	现货
100mg	￥5090.00	现货

CAS号：50-76-0纯度：99.51%

仅用于科研，不用于诊疗。未经明确授权不得转售。

021-55669583sales@apexbio.cnAPExBIO

特色产品

Annexin V-FITC/PI Apoptosis Kit

用于双染检测细胞凋亡和坏死

- 在10-20分钟内完成染色
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞

Phos binding reagent (Phosbind) acrylamide

分离和检测磷酸化/非磷酸化蛋白

- 新型的磷酸盐结合标签和功能分子
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白

Cell Counting Kit-8 (CCK-8)

用于细胞增殖和细胞毒性试验

- 比MTT，MTS或WST-1更敏感
- 对细胞无毒性
- 步骤更简单，无需有机溶剂

Cy5 TSA Fluorescence System Kit

Cy5荧光标记的酪胺信号放大系统

- 检测ICC/IHC/ISH中的低丰度靶点
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率

EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)

用于观察mRNA运送，定位，翻译

- 具有Cap1结构的荧光素酶mRNA
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰

HotStart™ 2X Green qPCR Master Mix

用于荧光定量目标DNA或cDNA

- 通过热启动机制优化扩增特异性
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单

HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus

高效的Cy5修饰RNA转录试剂盒

- 适合原位/Northernblot杂交实验
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量

产品描述产品性质产品应用操作说明产品引用文献摩尔浓度计算器

产品描述

Actinomycin D

产品性质

CAS号	50-76-0
分子式	C₆₂H₈₆N₁₂O₁₆
分子量	1255.43
SMILES	[H][C@@]12CCCN1C(=O)[C@H](NC(=O)[C@@H](NC(=O)C1=C3N=C4C(OC3=C(C)C=C1)=C(C)C(=O)C(N)=C4C(=O)N[C@H]1[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@]3([H])CCCN3C(=O)[C@H](NC1=O)C(C)C)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C2=O)C(C)C
信号通路	Apoptosis
存储条件	4°C干燥避光
运输条件	蓝冰

产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)

IC50和靶点

靶点	Apoptosis Inducers

操作说明

小分子化合物通用使用指南 Datasheet

APExBIO 顾客使用本产品发表的 67 篇科研文献

1. Yifan Hou, Qiqi Shen, et al. "A positive feedback loop between YTHDC1 and EP300 drives multiple myeloma tumorigenesis." Oncogene. 2026 Sep 25. PMID: 42791263

2. LinLin Yue, XiaoFeng Liu, et al. "circGAB1 Activates Autophagy to Promote Sepsis-Associated Acute Kidney Injury by Interacting with ELAVL1 to Activate the HMGB3/β-catenin Signaling Pathway." Appl Biochem Biotechnol. 2026 Jul 10. PMID: 42430057

3. Ting Lu, Yiying Yang, et al. "PUMILIO proteins maintain lineage balance via post-transcriptional repression of germline fate and promotion of somatic differentiation." Cell Rep. 2026 Jul 9;45(7):117682. PMID: 42424142

4. Xiaoning Huo, Ruihong Zhang, et al. "Linc00347 suppresses ferroptosis in drug-resistant gastric cancer cells through stabilizing FoxO6 mRNA mediated by YBX1." Int J Biol Macromol. 2026 May 18:152601. PMID: 42155744

5. Yalin Zhang, Jiangang Wang, et al. "CircCramp1l targets the miR-532-3p/HMGB1/Drp1 axis to regulate allergic rhinitis." Biochemical Pharmacology Volume 246, April 2026, 117699. PMID: 41519399

6. Zeyu Li, Wuxiyar Otkur, et al. "Glutamine homeostasis regulates PD-L1 level through STAT3 in pancreatic cancer." Genes & Diseases Available online 14 February 2026, 102092

7. Zhaohua Wang, Si Wen, et al. "FTO-mediated m6A modification regulates the osteogenic differentiation of ADSCs by targeting FOXO1." Stem Cell Res Ther. 2025 Dec 17;17(1):39. PMID: 41408314

8. Wenming Zhang, Rui Liu, et al. "The deubiquitylase OTUB1 drives gemcitabine resistance in pancreatic cancer by enhancing pyrimidine metabolism through modulating DHODH mRNA stability." Cell Death Dis. 2025 Oct 6;16(1):697. PMID: 41053036

9. Yibo Li, Zhaowen Su, et al. "Oligodendrocyte-Specific STAT5B Overexpression Ameliorates Myelin Impairment in Experimental Models of Parkinson’s Disease." Cells. 2025 Jul 25;14(15):1145. PMID: 40801579

10. Liangyan Wu, Lanlan Li, et al. "YTHDF2 regulates ACSL4-dependent ferroptosis of keratinocytes in diabetic wound healing." Clin Sci (Lond). 2025 Aug 5:CS20255877. PMID: 40785592

11. Jing Zhang, Chengming Gao, et al. "Long Non‐Coding RNA IGFRIL Couples with PTBP1 to Destabilize IGFBP3 mRNA to Promote the IGF1R‐AKT‐mTOR Axis and Hepatocellular Carcinoma." Adv Sci (Weinh). 2025 Oct;12(39):e07676. PMID: 40686314

12. Yifan Ya, Shuo Yang, et al. "m6A-methylated TAL1 exacerbates lipid accumulation in ethylene bisdithiocarbamate metabolite–induced anorectal malformations in rat fetuses via miR-205/LCOR pathway." Ecotoxicol Environ Saf. 2025 Jul 3:302:118594. PMID: 40614461

13. Jian Zhang, Pengfei Che, et al. "The m5C reader Ybx1 regulates embryonic cortical neurogenesis by promoting progenitor cell cycle progression." PLoS Biol. 2025 May 28;23(5):e3003175. PMID: 40435287

14. Yulu Zhang, Shunji Chen, Qiaosheng Wu et al. "The m6A reader YTHDF3 promotes TNBC progression by regulating CENPI stabilization." Front Oncol. 2025 May 8:15:1546723. PMID: 40406242

15. Chuanfeng Zhang, Jianing Wang, et al. "Circ‐Sirt6 promotes the stability and expression of m6A modified Sirt6 mRNA by recruiting IGF2BP2." FASEB J.2025 Feb 28;39(4):e70405. PMID: 39982754

16. Zhengzheng Li, Lei Chen, et al. "Loss of MNX1 Sensitizes Tumors to Cytotoxic T Cells by Degradation of PD‐L1 mRNA." Adv Sci (Weinh). 2025 Mar;12(12):e2403077. PMID: 39912421

17. Hongjuan Cao, Yue Yu, et al. "LIPUS promotes osteogenesis via the mcirc_9393-miR-217/326-SIRT1 axis in porous Ti6Al4V scaffolds." Bone Volume 201, December 2025, 117647

18. Wan Li, Jing Zhou, et al. "Lactylation of RNA m6A demethylase ALKBH5 promotes innate immune response to DNA herpesviruses and mpox virus." Proc Natl Acad Sci U S A. 2024 Oct 22;121(43):e2409132121. PMID: 39413129

19. Jiliang Tan, Jie Min, et al. "CircCHSY1 protects hearts against ischemia/reperfusion injury by enhancing heme oxygenase 1 expression via miR-24-3p." Cardiovasc Res. 2024 Jul 31:cvae162. PMID: 39082269

20. Fei Xie, Bin Liu, et al. "Smooth muscle NF90 deficiency ameliorates diabetic atherosclerotic calcification in male mice via FBXW7-AGER1-AGEs axis." Nat Commun. 2024 Jun 11;15(1):4985. PMID: 38862515

21. Zhouguang Wu, Bin Wang, et al. "Hsa_circ_0009096/miR-370-3p modulates hepatic stellate cell proliferation and fibrosis during biliary atresia pathogenesis." PeerJ. 2024 May 14:12:e17356. PMID: 38766485

22. Weiwei Liu, Wei Wang, et al. "CRISPR Screen Identifies the RNA-Binding Protein Eef1a1 as a Key Regulator of Myogenesis." Int J Mol Sci. 2024 Apr 28;25(9):4816. PMID: 38732031

23. Ye Ding, Meiqing Lin, et al. "RBM3 enhances the stability of MEF2C mRNA and modulates blood-brain barrier permeability in AD microenvironment." Biochim Biophys Acta Mol Cell Res. 2024 Jun;1871(5):119738. PMID: 38670534

24. Yajin Chen, et al. "CircNUP54 promotes hepatocellular carcinoma progression via facilitating HuR cytoplasmic export and stabilizing BIRC3 mRNA." Cell Death Dis. 2024 Mar 5;15(3):191. PMID: 38443362

25. Bingxi Lei, et al. "Depletion of the N6-Methyladenosine (m6A) reader protein IGF2BP3 induces ferroptosis in glioma by modulating the expression of GPX4." Cell Death Dis. 2024 Mar 1;15(3):181. PMID: 38429265

26. Yuanjian Shi, Zehao Pan, et al. "tRF-29-79 regulates lung adenocarcinoma progression through mediating glutamine transporter SLC1A5." Carcinogenesis. 2024 Feb 15:bgae010. PMID: 38366384

27. Zhenyan Miao, Jifei Li, et al. "Hsa_circ_0136666 stimulates gastric cancer progression and tumor immune escape by regulating the miR-375/PRKDC Axis and PD-L1 phosphorylation." Mol Cancer. 2023 Dec 13;22(1):205. PMID: 38093288

28. Yiyi Ji, Weiwei Zhang, et al. "The ELAVL3/MYCN positive feedback loop provides a therapeutic target for neuroendocrine prostate cancer." Nat Commun. 2023 Nov 28;14(1):7794. PMID: 38016952

29. Haopei Zhang, Qiuyue Chen, et al. "DDX24 Mutation Alters NPM1 Phase Behavior and Disrupts Nucleolar Homeostasis in Vascular Malformations." Int J Biol Sci. 2023 Aug 6;19(13):4123-4138. PMID: 37705750

30. Jin Fan, Mengqi Zhuang, et al. "RNA N6-methyladenosine reader IGF2BP3 promotes acute myeloid leukemia progression by controlling stabilization of EPOR mRNA." PeerJ. 2023 Aug 30:11:e15706. PMID: 37663284

31. Huihui Bai, Meina Jiang, et al. "Whole Blood-Derived circUSP10 Acts as a Diagnostic Biomarker in Patients With Early-Stage Non-Small-Cell Lung Cancer." Cell Transplant. 2023 Jan-Dec;32:9636897231193066. PMID: 37632352

32. Jianye Zhong, Abai Xu, et al. "Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p." Cell Death Discov. 2023 Aug 2;9(1):283. PMID: 37532687

33. Jia-Jie Lin, Rui Chen, et al. "Hsa_circ_0001402 alleviates vascular neointimal hyperplasia through a miR-183-5p-dependent regulation of vascular smooth muscle cell proliferation, migration, and autophagy." J Adv Res. 2023 Jul 25;S2090-1232(23)00201-1. PMID: 37499939

34. Xiaoxiao Ouyang, Xueyun Wang, et al. "Bacterial effector restricts liquid-liquid phase separation of ZPR1 to antagonize host UPRER." Cell Rep. 2023 Jul 25;42(7):112700. PMID: 37379216

35. Xia Yang, Qiaorui Bai, et al. "m6A‐Dependent Modulation via IGF2BP3/MCM5/Notch Axis Promotes Partial EMT and LUAD Metastasis." Adv Sci (Weinh). 2023 Jul;10(20):e2206744. PMID: 37171793

36. Shi D, Liu X, et al. "Yth m6A RNA-Binding Protein 1 Regulates Osteogenesis of MC3T3-E1 Cells under Hypoxia via Translational Control of Thrombospondin-1." Int J Mol Sci 2023 Jan 16;24(2) PMID: 36675257

37. Jinrui Zhang, Ge Zhang, et al. "Loss of RBMS1 promotes anti-tumor immunity through enabling PD-L1 checkpoint blockade in triple-negative breast cancer." Cell Death Differ. 2022 May 10. PMID: 35538152

38. Nan Zhang, Yan Shen, et al. "The m6A reader IGF2BP3 promotes acute myeloid leukemia progression by enhancing RCC2 stability." Exp Mol Med. 2022 Feb;54(2):194-205. PMID: 35217832

39. Xin Lin, Liwen Zhou, et al. "RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53." J Biol Chem. 2021 Dec 22;101524. PMID: 34953860

40. Ye Ding, Xiaobai Liu, et al. "Pseudogene RPL32P3 regulates the blood–tumor barrier permeability via the YBX2/HNF4G axis." Cell Death Discov. 2021 Nov 24;7(1):367. PMID: 34819492

41. Diefei Liang, Wei-Jye Lin, et al. "m6A reader YTHDC1 modulates autophagy by targeting SQSTM1 in diabetic skin." Autophagy. 2021 Oct 17;1-20. PMID: 34657574

42. Yi‑tian Chen, Dan Xiang, et al. "Upregulation of lncRNA NIFK-AS1 in hepatocellular carcinoma by m 6 A methylation promotes disease progression and sorafenib resistance." Hum Cell. 2021 Nov;34(6):1800-1811. PMID: 34374933

43. Yiping Zhu, Fang Wu, et al. "A positive feedback regulatory loop involving the lncRNA PVT1 and HIF-1α in pancreatic cancer." J Mol Cell Biol. 2021 Dec 6;13(9):676-689. PMID: 34245303

44. Wen Li, Shanshan Wang, et al. "CircHECTD1 Regulates Cell Proliferation and Migration by the miR-320-5p/SLC2A1 Axis in Glioblastoma Multiform." Front Oncol. 2021 May 17;11:666391. PMID: 34079759

45. Duolan Naren, Tianyou Yan, et al. "High Wilms' tumor 1 associating protein expression predicts poor prognosis in acute myeloid leukemia and regulates m 6 A methylation of MYC mRNA." J Cancer Res Clin Oncol. 2021 Jan;147(1):33-47. PMID: 32880751

46. Yiming Xu, Dandan Lv, et al. "METTL3 Promotes Lung Adenocarcinoma Tumorigenesis and Inhibits Ferroptosis by Stabilizing SLC7A11 m6A Modification." Research Square. rs-1085921/v1 21203/rs.3.rs-1085921/v1

47. Dandan Zhang, Ni Ni, et al. "CircRNA-vgll3 promotes osteogenic differentiation of adipose-derived mesenchymal stem cells via modulating miRNA-dependent integrin α5 expression." Cell Death Differ 2020 Aug 19. PMID: 32814879

48. Hongyu Yang, Qingxia Lin, et al. "LncRNA NR_030777 Alleviates Paraquat-induced Neurotoxicity by Regulating Zfp326 and Cpne5." Toxicol Sci. 2020 Nov 1;178(1):173-188. PMID: 32382014

49. Dai J, Yang L, et al. "A Functional Synonymous Variant in PDGFRA Is Associated with Better Survival in Acral Melanoma." J Cancer. 2020;11(10):2945-2956. Published 2020 Mar 4. PMID: 32226509

50. Bahat A, Lahav O, et al. "Targeting Spt5-Pol II by Small-Molecule Inhibitors Uncouples Distinct Activities and Reveals Additional Regulatory Roles." Mol Cell. 2019 Sep 25. pii: S1097-2765(19)30663-X. PMID: 31564557

51. Luo Y, Teng X, et al. "CD146-HIF-1α hypoxic reprogramming drives vascular remodeling and pulmonary arterial hypertension." Nat Commun. 2019 Aug 7;10(1):3551. PMID: 31391533

52. Xiao Y, Pan J, et al. "LncRNA MALAT1 increases the stemness of gastric cancer cells via enhancing SOX2 mRNA stability." FEBS Open Bio. 2019 Jul;9(7):1212-1222. PMID: 31037832

53. Wu H, He Y, et al. "LncRNA THOR increases osteosarcoma cell stemness and migration by enhancing SOX9 mRNA stability." FEBS Open Bio. 2019 Mar 20;9(4):781-790. PMID: 30984551

54. Gong F, Dong D, et al. "Long non-coding RNA FENDRR attenuates the stemness of non-small cell lung cancer cells via decreasing multidrug resistance gene 1 (MDR1) expression through competitively binding with RNA binding protein HuR." Eur J Pharmacol. 2019 Jun 15;853:345-352. PMID: 30981768

55. Cao H, Yu D, et al. "Hypoxia destroys the microstructure of microtubules and causes dysfunction of endothelial cells via the PI3K/Stathmin1 pathway." Cell Biosci. 2019 Feb 18;9:20. PMID: 30820314

56. Oladimeji PO, Wright WC, et al. "RNA interference screen identifies NAA10 as a regulator of PXR transcription." Biochem Pharmacol. 2018 Dec 16;160:92-109. PMID: 30566892

57. Bao L, Yuan L, et al. "A FUS-LATS1/2 Axis Inhibits Hepatocellular Carcinoma Progression via Activating Hippo Pathway." Cell Physiol Biochem. 2018;50(2):437-451. PMID: 30308519

58. Song H, Xu Y, et al. "LncRNA THOR increases the stemness of gastric cancer cells via enhancing SOX9 mRNA stability." Biomed Pharmacother. 2018 Sep 15;108:338-346. PMID: 30227327

59. Rahnamoun H, Lee et al. "RNAs interact with BRD4 to promote enhanced chromatin engagement and transcription activation." Nat Struct Mol Biol. 2018 Aug;25(8):687-697. PMID: 30076409

60. Wang X, Hu H, et al. "RNA binding protein Lin28B confers gastric cancer cells stemness via directly binding to NRP-1." Biomed Pharmacother. 2018 May 19;104:383-389. PMID: 29787985

61. WEICHENG PAN, JINHUI PANG, et al. "RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR-142-3p/HMGA1 axis." ONCOLOGY LETTERS. 2018 May 31

62. Caihua Wang, Yonghong Zhang, et al. "miR-204 enhances p27 mRNA stability by targeting Brd4 in head and neck squamous cell carcinoma." Oncology Letters. July 19, 2018

63. Yang L, Zhang Y, et al. "RNPC1 inhibits non-small cell lung cancer progression via regulating miR-181a/CASC2 axis." Biotechnol Lett. 2017 Dec 29. PMID: 29288351

64. Wang Z, Pang J, et al. "RNA binding protein Lin28A promotes osteocarcinoma cells progression by associating with the long noncoding RNA MALAT1." Biotechnol Lett. 2017 Dec 4. PMID: 29204769

65. Xu W, Gong F, et al. "RNA-binding protein Dnd1 inhibits epithelial-mesenchymal transition and cancer stem cell-related traits on hepatocellular carcinoma cells." Biotechnol Lett. 2017 Jun 7. PMID: 28593479

66. Zhang Z, Huang A, et al. "HuR promotes breast cancer cell proliferation and survival via binding to CDK3 mRNA." Biomed Pharmacother. 2017 May 10;91:788-795. PMID: 28501005

67. Xu CZ, Jiang C, et al. "A Feed-Forward Regulatory Loop between HuR and the Long Noncoding RNA HOTAIR Promotes Head and Neck Squamous Cell Carcinoma Progression and Metastasis." Cell Physiol Biochem.2016;40(5):1039-1051. PMID: 27941336

摩尔浓度计算器

质量

= 浓度

x 体积

x 分子量

*在配置溶液时，请务必参考产品标签上、MSDS / COA（可在Apexbio的产品页面获得）批次特异的分子量使用本工具。

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