WST-1 Cell Proliferation Colorimetric Assay Kit
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Cell proliferation can be detected by a variety of methods. Cell proliferation induces an increase in the activity of the mitochondrial dehydrogenases, which cleaves the tetrazolium salt WST-1 to formazan.
The WST-1 Cell Proliferation Colorimetric Assay Kit provides a fast and sensitive way for quantification of cell proliferation and viability. Cell proliferation causes the increase in the amount of formazan dye formed that can be quantified by measuring the absorbance of the dye solution at 440 nm using microtiter plate reader. The kit can be used for the analysis of cell proliferation in response to pharmaceutical compounds, cytotoxic compounds like anticancer drugs and many other toxic agents. The assay can also be used for the detection of cell proliferation in response to cytokines, mitogens, growth factors and nutrients, etc. The assay is very easy and simple, requiring no solubilization, no washing and no harvesting steps, and is faster and more sensitive than MTS, MTT or XTT-based assays. The entire assay can be performed in a microtiter plate.
| Features | Fast and convenient Simple one-step procedure; takes less than 4 hours. The method is so easy that it requires no washing/harvesting/or solubilization steps and is faster and more sensitive than MTT/XTT/or MTS-based assays. The entire assay can be performed directly in a 96-well plate. |
| Shipping | Gel pack |
| Storage Conditions | Store at -20°C. |
Related Biological Data
Related Biological Data
Related Biological Data
| Kit components | WST-1 Developer Reagent Electron Mediator Solution |
| Detection method | Absorbance (420-480 nm) |
| Sample type | Cell culture (adherent and suspension) |
| Species reactivity | Mammalian |
| Applications | This fast and sensitive cell proliferation assay uses a water-soluble tetrazolium salt WST-1. The assay measures cell proliferation in response to growth factors/cytokines/mitogens/and nutrients etc. |