Trichostatin A (TSA)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Trichostatin A (TSA)是一种有效的组蛋白去乙酰化酶（HDAC）抑制剂，也是一种抗真菌类抗生素，具有抑制细胞生长和诱导细胞分化的特性。在培养的哺乳动物细胞以及分离的细胞核提取物中，TSA在低浓度时可以非竞争性并可逆地抑制HDAC。TSA能够使细胞停滞在G1和G2期，诱导细胞分化，恢复培养细胞的转化形态。据报道，在人乳腺癌细胞系中，TSA抑制乳腺癌细胞的增值（IC50值为 124.4 ± 120.4 nM)，并导致组蛋白H4的超乙酰化。
参考文献：
David M. Vigushin, Simak Ali, Paul E. Pace, Nina Mirsaidi, Kazuhiro Ito, Ian Adcock, and R. Charles Coombes. Trichostatin A is a histone deacetylase inhibitor with potent antitumot activity against breast cancer in vivo. Clin Cancer Res 2001;7:971-976
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| Storage | Desiccate at -20°C |
| M.Wt | 302.37 |
| Cas No. | 58880-19-6 |
| Formula | C17H22N2O3 |
| Synonyms | Trichostatin A,TSA |
| Solubility | insoluble in H2O; ≥15.12 mg/mL in DMSO; ≥16.56 mg/mL in EtOH with ultrasonic |
| Chemical Name | (2E,4E,6R)-7-[4-(dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxohepta-2,4-dienamide |
| SDF | Download SDF |
| Canonical SMILES | CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验[1]： | |
|
细胞系 |
人乳腺癌细胞系 |
|
溶解方法 |
在DMSO中的溶解度>10 mM。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
反应条件 |
96 h；10 μM的TSA溶解于含0.1％（体积/体积）乙醇的生长培养基中。 |
|
应用 |
TSA抑制8个乳腺癌细胞系的增殖，平均IC50值为124.4 ± 120.4 nM（范围：26.4-308.1 nM）。TSA处理导致明显的组蛋白H4的超乙酰化。 |
| 动物实验[1]： | |
|
动物模型 |
NMU诱导的肿瘤异种移植自交系处女雌性（Ludwig/Wistar/Olac）大鼠 |
|
剂量 |
500 μg/kg/day，4周；注射给药 |
|
应用 |
TSA在体内具有明显的抗肿瘤活性。TSA的抗肿瘤活性归因于分化诱导。 |
|
注意事项 |
请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的，属于正常现象。 |
|
References: 1. Vigushin DM1, Ali S, Pace PE et al. Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo. Clin Cancer Res. 2001 Apr;7(4):971-6. |
|
| Targets | HDAC | |||||
| IC50 | ~1.8 nM |
质量控制和MSDS
- 批次：
化学结构
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