Tn5 Transposase
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Tn5 Transposase是野生型Tn5转座酶的高活性突变体，可以在体外高效地将Tn5转座子插入到目标序列。Tn5 Transposase特异性识别Tn5转座子两端反向重复的mosaic end（ME）序列，Tn5 Transposase体外转座插入效率比野生型Tn5转座酶高1000倍。
Tn5 Transposase的插入位点具有很高的随机性，可将含有成对识别序列的双链DNA片段随机整合到宿主细胞的基因组中（体外转基因操作），此外，Tn5 Transposase常应用于二代测序建库等研究领域。
1. 一管式酶促反应，DNA片段化和接头连接等同时进行，操作简便
2. 高活性： DNA片段化活性极高
3. 各组分均经过了严格的质量控制和功能验证，保真度高、稳定性强、重复性好
| Components | 20 μL | 100 μL |
| Tn5 Transposase (400 ng/μL)* | 20 μL | 100 μL |
| Annealing Buffer | 400 μL | 1 mL x 2 |
| Coupling Buffer | 200 μL | 1 mL |
| 5X Reaction Buffer | 200 μL | 1 mL |
Tn5 Transposase储存于-80°C，其他组分储存于-20°C。 构建的转座复合体于-20°C保存，有效期1年。 *Note: Tn5 Transposase质量浓度为400 ng/μL，换算为摩尔浓度为7.5 pmol/μL。 | ||