Terminal Transferase
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
末端脱氧核苷酸转移酶(terminal deoxynucleotidyl transferase, TdT)是一种非模板依赖性的DNA聚合酶,可以催化脱氧核苷酸重复加成到DNA分子(双链/单链DNA或寡核苷酸链)的3'羟基端,可以催化的寡核苷酸的最短长度为3个核苷酸。当模板是RNA链时,最终TdT的催化活性依赖于模板3'的核苷酸特点和高级结构,但通常,RNA模板下的合成效果低于DNA模板的。
本试剂盒常用于合成同聚物和杂聚物、线性化的双链DNA 3’-OH端同聚物加尾、寡聚脱氧核苷酸和DNA标记(e.g., ddNTP, DIG-dUTP, fluorescein-12-dutp等)、5’-RACE(快速扩增cDNA末端)及凋亡的原位定位等。
Component | Size |
Terminal Deoxynucleotidyl Transferase (20 U/µl) | 25 μl |
5×Reaction Buffer | 0.4 ml |
Long-term storage: -20 °C |