Resiquimod (R-848)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Resiquimod (R-848, S-27609)是一种免疫应答调节剂 [1]。
Resiquimod (R-848, S-27609) 是Imiquimod (R-837, S-26308) 的类似物。据报道,在人外周血单核细胞(PBMCs)中,Resiquimod呈剂量依赖性地诱导某些细胞因子,包括IFN、TNF、IL-1β和IL-6。此外,Resiquimod刺激细胞内IL-1β增加约15%。实验结果表明,单核细胞和B细胞均产生IFN以响应Resiquimod。另外,与未处理的PBMC相比,Resiquimod增加IFN-α、TNF和IL-8 mRNA表达。Resiquimod在动物模型中还是一种有效的抗病毒和抗肿瘤药物 [1]。
参考文献:
[1] Gibson SJ1, Imbertson LM, Wagner TL, Testerman TL, Reiter MJ, Miller RL, Tomai MA. Cellular requirements for cytokine production in response to the immunomodulators imiquimod and S-27609. J Interferon Cytokine Res. 1995 Jun; 15(6):537-45.
- 1. Hua Zhang, Wei-Lun Tang, et al. "Development of thermosensitive resiquimod-loaded liposomes for enhanced cancer immunotherapy." J Control Release. 2020 Nov 13;S0168-3659(20)30662-3. PMID:33189786
- 2. Mohammed S, Vineetha NS, et al. "Examination of the role of sphingosine kinase 2 in a murine model of systemic lupus erythematosus." FASEB J. 2019 Jun;33(6):7061-7071. PMID:30840833
- 3. Qu S, Qin T, et al. "The effects of resiquimod in an ovalbumin-induced allergic rhinitis model." Int Immunopharmacol. 2018 Jun;59:233-242. PMID:29665497
Storage | Store at -20°C |
M.Wt | 314.38 |
Cas No. | 144875-48-9 |
Formula | C17H22N4O2 |
Solubility | insoluble in H2O; ≥12.65 mg/mL in EtOH with ultrasonic; ≥15.85 mg/mL in DMSO |
Chemical Name | 1-[4-amino-2-(ethoxymethyl)imidazo[4,5-c]quinolin-1-yl]-2-methylpropan-2-ol |
SDF | Download SDF |
Canonical SMILES | CCOCC1=NC2=C(N1CC(C)(C)O)C3=CC=CC=C3N=C2N |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
源自野生型和MyD88缺陷型小鼠的腹膜巨噬细胞 |
制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液,一般步骤如下:请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20°C可放置数月。 |
反应条件 |
100 nM |
实验结果 |
与LPS诱导的激活(不依赖MyD88)不同,Resiquimod诱导的NF-κB激活完全依赖于MyD88。据推测,Resiquimod通过TLR激活巨噬细胞。 |
动物实验 [1]: | |
动物模型 |
野生型、TLR7缺陷型和MyD88缺陷型小鼠 |
给药剂量 |
50 nmol;腹腔注射 |
实验结果 |
在野生型小鼠中,Resiquimod(50 nmol,腹腔注射)增加细胞因子(包括IFN-α、TNF-α和IL-12)的血清浓度。然而,在TLR7缺陷型和MyD88缺陷型小鼠中,上述细胞因子没有增加。 |
其它注意事项 |
请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同,但仍处于实验系统误差的允许范围内。 |
References: [1]. Hemmi H, Kaisho T, Takeuchi O, Sato S, Sanjo H, Hoshino K, Horiuchi T, Tomizawa H, Takeda K, Akira S. Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway. Nat Immunol. 2002 Feb;3(2):196-200. |
质量控制和MSDS
- 批次: