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Recombinant Protein G

 
Catalog No.
P2002
可溶性重组蛋白G(Protein G)
组合的产品项目
规格价格库存 数量
5mg
¥ 272.00
现货
10mg
¥ 409.00
现货
100mg
¥ 1,454.00
现货

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A

Description

天然Protein G是一种细菌蛋白,来源于某些b型溶血性链球菌的细胞壁。它与不同种类的免疫球蛋白的Fc部分有高度的亲和力。G蛋白与人类、小鼠和大鼠的所有IgG亚类结合。它还能与豚鼠、兔、山羊、牛、绵羊和马的总IgG结合。G蛋白优先与IgG的Fc部分结合,但也能与Fab区域结合,因此它对纯化IgG的F(ab')片段很有用。由于它对许多哺乳动物免疫球蛋白的Fc区有亲和力,蛋白G被认为是生物化学和免疫学的通用试剂。与葡萄球菌蛋白A相比,蛋白G与IgG亚类的结合范围更广(具体参数见Table 1)。

本产品为经过优化过的具有更高稳定性的Protein G可溶性重组蛋白,分子量约24.37 kDa(SDS-PAGE)。Protein G与IgG在pH 7.4时有最佳结合,同时在中性或者生理条件下(pH 7.0-7.6)也能很好的结合(具体参数见Table 2)。

Species

Subclass

Protein A binding

Protein G binding

Human

lgA

++

-

lgD

++

-

IgE

++

-

lgG1

++++

++++

lgG2

++++

++++

IgG3

-

++++

lgG4

++++

++++

lgM

++

-

Mouse

IgG1

+

++++

lgG2a

++++

++++

lgG2b

+++

+++

lgG3

++

+++

lgM

+/-

-

Rat

lgG1

-

+

lgG2a

-

++++

lgG2b

-

++

lgG3

+

++

Avian egg yolk

IgY

-

-

Cow

 

++

++++

Dog

 

++

+

Goat

 

-

++

Guinea Pig

lgG1

++++

++

lgG2

++++

++

Hamster

 

+

++

Horse

 

++

++++

Koala

 

-

+

Llama

 

-

+

Monkey (rhesus)

 

++++

++++

Pig

 

+++

+++

Rabbit

 

++++

+++

Sheep

 

+/-

++

 

Table 1 Affinity data for Protein A and Protein G for different sources and subtypes of IgG. ++++ = Strong Binding; ++~+++ = Medium Binding; + = Weak Binding; +/- = Weak or No Binding; - = No Binding.

Name

Recombinant Protein G

Nature

Recombinant

Purity

>99.9% by SDS-PAGE and HPLC analyses

Endotoxin level

< 0.100 EU/μg determined by LAL method

Expression system

E.coli

MW

24.37 kDa

Tags

6x His-tag on N-terminus.

Species

Human, Rabbit

State

Liquid

Constituents

Supplied as a 0.22 μm filtered solution of 1x PBS, pH 8.2.

Concentration

5 mg/mL

Reconstitution

Dissolve in distilled water or saline.

PI

~5.01

Sequence

The sequence cannot be shared.

Table 2 Detailed parameters of Recombinant Protein G.

质量控制

质量控制和DataSheet

批次:

相关生物数据

Recombinant Protein G
 

Components and Storage

Components P2002-5 mg P2002-10 mg P2002-100 mg
Recombinant Protein G 1 mL 2 mL 20 mL

Store the components dry at -80 °C for 18 months.