Recombinant Mouse M-CSF, Tag Free
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M- CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.
参考文献:
1. Pandit J, Bohm A, Jancarik J, Halenbeck R, Koths K, Kim SH. Three-dimensional structure of dimeric human recombinant macrophage colony-stimulating factor. Science. 1992 Nov 20;258(5086):1358-62. doi: 10.1126/science.1455231. PMID: 1455231.
2. Tokai M, Kawasaki H, Kikuchi Y, Ouchi K. Cloning and characterization of the CSF1 gene of Saccharomyces cerevisiae, which is required for nutrient uptake at low temperature. J Bacteriol. 2000 May;182(10):2865-8. doi: 10.1128/jb.182.10.2865-2868.2000. PMID: 10781556; PMCID: PMC101996.
3. Fan X, Fan D, Gewant H, Royce CL, Nanes MS, Rubin J. Increasing membrane-bound MCSF does not enhance OPGL-driven osteoclastogenesis from marrow cells. Am J Physiol Endocrinol Metab. 2001 Jan;280(1):E103-11. doi: 10.1152/ajpendo.2001.280.1.E103. PMID: 11120664.
别称 | C87615 Protein, Mouse; Csfm Protein, Mouse; MCSF Protein, Mouse; op Protein, Mouse. |
Gene ID | 12977 |
Accession # | P07141-1 |
蛋白序列 | Met1-Glu262 |
分子量 | 26 kDa |
来源 | 293T cells |
生物活性 | 以下数据来自于小鼠CTLL-2细胞的剂量依赖性实验: ED50:< 0.1 ng/mL 放射性比度:> 1x107units/mg. |
产品形式 | 该蛋白以溶解于PBS缓冲液的形式提供。 |
存储说明 | 此产品可稳定存在于如下条件中: 4°C保存一周; -20°C保存3个月。 请避免多次反复冻融。 |