Recombinant Human IGF-1, Tag Free
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Insulin-like growth factor I (IGF-1) is a polypeptide endocrine hormone structurally similar to insulin and is mainly produced in the liver when stimulated by growth hormone. IGF-1 is a growth factor that stimulates the proliferation of various cell types including muscle, bone, and cartilage tissue. IGF-1 binds and interacts with the IGF-1 binding proteins (IGFBPs). IGF-1 binds to the IGF-1 receptor with a higher affinity than the insulin receptor.
Human IGF-I is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides, which are proteolyzed to produce the identical 7.6 kDa mature IGF-I. Mature human IGF I shares 94% and 96% aa sequence identity with mouse IGF1 and rat IGF1, respectively, and exhibits cross species activity. Human IGF-1 is a 7.6 kDa protein containing 70 amino acid residues. The recombinant human IGF-1 was produced using animal origin free technology.
参考文献:
1. Zhao Y, Wang Q, et al. "Glutamine protects against oxidative stress injury through inhibiting the activation of PI3K/Akt signaling pathway in parkinsonian cell model." Environ Health Prev Med. 2019 Jan 5;24(1):4.
别称 | IGF1A, Insulin-Like Growth Factor 1, Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF. | Gene ID | 3479 | Accession # | P05019 |
蛋白序列 | Gly49-Ala118 |
分子量 | 7.6 kDa |
来源 | 293T细胞 |
生物活性 | 以下数据来自于小鼠CTLL-2细胞的剂量依赖性实验: ED50:< 0.1 ng/mL 放射性比度:> 1x107units/mg. |
产品形式 | 该蛋白以溶解于PBS缓冲液的形式提供。 |
存储说明 | 此产品可稳定存在于如下条件中: 4°C保存一周; -20°C保存3个月。 请避免多次反复冻融。 |