RA-9
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
RA-9是一种有效的且具有选择性和细胞渗透性的蛋白酶体相关DUBs抑制剂。用RA-9治疗可选择性杀死卵巢癌细胞，包括来自患者肿瘤的原代细胞，导致ES-2和TOV-21G卵巢癌细胞中p-eIF2-α水平的时间依赖性增加，印证了药物治疗后细胞转化机制的普遍停止。RA-9的暴露，通过未溶解的未折叠蛋白反应（UPR），包括激活内质网应激反应，诱导了细胞凋亡。使用带有人类卵巢癌腹膜内异种移植模型的免疫缺陷小鼠进行的临床前研究显示，GRP-78和高分子量聚泛素化蛋白水平增加，表明RA-9治疗可延缓肿瘤生长并提高总体存活率，并对宿主无明显毒性。
1. Coughlin K, et al. Small-molecule RA-9 inhibits proteasome-associated DUBs and ovarian cancer in vitro and in vivo via exacerbating unfolded protein responses. Clin Cancer Res. 2014;20(12):3174-3186.
| Storage | Store at -20°C |
| M.Wt | 365.34 |
| Cas No. | 919091-63-7 |
| Formula | C19H15N3O5 |
| Solubility | ≥9.2 mg/mL in DMSO with ultrasonic |
| Chemical Name | (3E,5E)-3,5-bis(4-nitrobenzylidene)piperidin-4-one |
| SDF | Download SDF |
| Canonical SMILES | O=C1/C(CNC/C1=C\C2=CC=C([N+]([O-])=O)C=C2)=C/C3=CC=C([N+]([O-])=O)C=C3 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
