Phalloidin-Fluorescein Conjugate
Catalog No.
C4340
绿色荧光标记鬼笔环肽探针,用于检测丝状肌动蛋白(F-actin)
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Phalloidin-Fluorescein Conjugate是由荧光素(Fluorescein)与鬼笔环肽(货号B7678)偶联的绿色鬼笔环肽荧光探针,用于标记丝状肌动蛋白(F-actin)。荧光素提供绿色荧光(Ex / Em max = 492/518 nm)。
Phalloidin(鬼笔环肽)是一种来源于毒蕈类鬼笔鹅膏(Amanita phalloides)的环状七肽毒素,以高亲和力(Kd= 20 nM)选择性地结合丝状肌动蛋白(F-actin),而不会与单体肌动蛋白(G-actin)结合[1]。肌动蛋白以两种形式存在,即单体和多聚体。肌动蛋白单体为球形,其表面上有一ATP结合位点。肌动蛋白单体一个接一个连成一串肌动蛋白链,两串这样的肌动蛋白链互相缠绕扭曲成一股微丝。这种肌动蛋白多聚体又被称为丝状肌动蛋白。鬼笔环肽与丝状肌动蛋白(微丝)的结合,阻止了微丝的解离,稳定了微丝结构[2],破坏了微丝的聚合和解聚的动态平衡。
将荧光染料与鬼笔环肽进行偶联,偶联物常用在组织切片、固定细胞、透化的细胞和无细胞的实验中选择性地标记 F-actin,广泛应用于微丝骨架在细胞中的成像[3]。标记后的鬼笔环肽对大细丝和小细丝具有相似的亲和力,无论是动植物来源的肌肉细胞或非肌肉细胞,按照每一个肌动蛋白亚基约与一个鬼笔环肽分子的计量比结合。这种成像优于抗体染色:鬼笔环肽无物种限制,且几乎不存在非特异性染色,染色区和非染色区域对比极其明显。因此,鬼笔环肽衍生物特别适合替代肌动蛋白(Actin)抗体进行相关研究。由于它们不与单体 G-actin发生结合,这与某些抗肌动蛋白的抗体有所不同。
References:
[1] Estes JE, Selden LA, Gershman LC. Mechanism of action of phalloidin on the polymerization of muscle actin. Biochemistry. 1981 Feb 17;20(4):708-12.
[2] Oda T, Namba K, Maéda Y. Position and orientation of phalloidin in F-actin determined by X-ray fiber diffraction analysis. Biophys J. 2005;88(4):2727–2736.
[3] Melak M, Plessner M, Grosse R. Actin visualization at a glance. J Cell Sci. 2017 Feb 1;130(3):525-530.
Physical Appearance | A solution in DMSO (1000X). To change the solvent, simply evaporate the DMSO (1000X) under a gentle stream of nitrogen and immediately add the solvent of choice. |
Storage | Store at -20°C |
M.Wt | 1146.18 |
Formula | C56H59N9O16S |
Solubility | Soluble in DMSO |
SDF | Download SDF |
Canonical SMILES | OC(C)(CNC(C1=CC(C(O)=O)=C(C=C1)C2=C(C(OC3=C2C=CC(O)=C3)=C4)C=CC4=O)=O)CC(NC(C5NC(C(C)NC(C6CC(CN67)O)=O)=O)=O)C(NC(C)C(NC(C(NC(CSC8=C(C9=C(N8)C=CC=C9)C5)C7=O)=O)C(C)O)=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |