Dihydrorhodamine 123
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Dihydrorhodamine 123(二氢罗丹明123,DHR 123)是罗丹明123的还原形式,是一种常用的荧光线粒体染料[1]。二氢罗丹明123本身是不发荧光的,它能够很容易穿透细胞膜进入细胞,并被细胞内的氧化性物质或氧化还原系统氧化成可发荧光的罗丹明123,后者聚集在线粒体膜上,发射亮绿色荧光(Ex/Em=500/536 nm)。单一的一氧化氮(NO),超氧化物,或过氧化氢(H2O2)不能氧化二氢罗丹明123,但是,当这些活性氧物质与其他细胞组分比如细胞色素c氧化酶或Fe2+联合起来能够氧化DHR 123生成罗丹明123。荧光信号可以通过荧光计,流式细胞仪或荧光显微镜检测。
二氢罗丹明123可用于检测活性氧(ROS),包括超氧化物(在过氧化物酶或细胞色素c存在下)和过氧亚硝酸盐。二氢罗丹明123广泛地用于多种细胞,例如人中性粒细胞[2]、肺肿瘤SPC-A-1细胞[3]、内皮细胞[4]、HaCaT 细胞、嗜酸性细胞、鼠肥大细胞、几内亚猪中性粒细胞、软骨细胞、鼠近端肾小管细胞等。
References:
[1] Yazdani M. Concerns in the application of fluorescent probes DCDHF-DA, DHR 123 and DHE to measure reactive oxygen species in vitro. Toxicol In Vitro. 2015 Dec 25;30(1 Pt B):578-82.
[2] Rothe G, Oser A, Valet G. Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes. Naturwissenschaften. 1988 Jul;75(7):354-5.
[3] Qin Y, Lu M, Gong X. Dihydrorhodamine 123 is superior to 2,7-dichlorodihydrofluorescein diacetate and dihydrorhodamine 6G in detecting intracellular hydrogen peroxide in tumor cells. Cell Biol Int. 2008 Feb;32(2):224-8. Epub 2007 Sep 7.
[4] Royall JA, Ischiropoulos H. Evaluation of 2',7'-dichlorofluorescin and dihydrorhodamine 123 as fluorescent probes for intracellular H2O2 in cultured endothelial cells. Arch Biochem Biophys. 1993 May;302(2):348-55.
Physical Appearance | crystalline solid |
Storage | Store at -20°C |
M.Wt | 346.38 |
Cas No. | 109244-58-8 |
Formula | C21H18N2O3 |
Synonyms | DHR 123 |
Solubility | ≤10mg/ml in ethanol;10mg/ml in DMSO;10mg/ml in dimethyl formamide |
Chemical Name | methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate |
SDF | Download SDF |
Canonical SMILES | COC(C1=CC=CC=C1C(C2=C3C=C(N)C=C2)C4=C(O3)C=C(N)C=C4)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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