Green CMFDA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Green CMFDA(5-氯甲基荧光素二乙酸酯)是一种具有细胞膜渗透性可对细胞进行示踪的硫醇反应性(thiol-reactive)荧光染料。CMFDA是二乙酸荧光素(Fluorescein diacetate,FDA)的氯甲基衍生物,本身没有荧光。当其穿透细胞膜进入活细胞后,CMFDA中的亲脂性基团可被胞浆内酯酶水解,生成5-氯甲基荧光素(5-chloromethylfluorescein);5-氯甲基荧光素可发出绿色荧光(Ex=492nm,Em=517nm),后者因带电荷不能自由穿透细胞膜而留在细胞内,然后可以与细胞内含硫醇的蛋白质和肽反应形成醛固定的结合物。该探针也可以首先与细胞内含硫醇的生物分子反应,但是结合物是非荧光的,直到其乙酸酯被除去。
CMFDA具有理想的“跟踪”特性,荧光非常稳定,无毒(工作浓度范围内),可以在活细胞中保留数代(通常三到六代),可以多代跟踪细胞运动。CMFDA稳定标记的时间至少72小时。在细胞分裂增殖过程中,CMFDA标记荧光可分配到两个子代细胞中,且几乎不会使邻近细胞染色(有极少情况下通过细胞间缝隙连接发生染色)。
CMFDA最适合与红色荧光染料和蛋白质共染,如红色荧光蛋白(red fluorescent protein,RFP),橙色CMRA和CMRA染料[1,2]。CMFDA通常被用来标记干细胞[3,4]。
References:
[1] Trontelj K, Usaj M, Miklavcic D. Cell electrofusion visualized with fluorescence microscopy. J Vis Exp. 2010;(41):1991. Published 2010 Jul 1.
[2] Yang XP, Gallo M, Ngan I, et al. Use of CMFDA and CMTMR fluorescent dyes in FACS-based antibody screening. Biotechniques. 2002 Mar;32(3):678-80, 682.
[3] Bergenheim F, Seidelin JB, Pedersen MT, et al. Fluorescence-based tracing of transplanted intestinal epithelial cells using confocal laser endomicroscopy. Stem Cell Res Ther. 2019;10(1):148. Published 2019 May 27.
[4] Andrzejewska, A., Jablonska, A., Seta, M. et al. Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity. Stem Cell Res Ther 10, 187 (2019).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 464.85 |
Cas No. | 136832-63-8 |
Formula | C25H17ClO7 |
Solubility | Soluble in DMSO |
Chemical Name | 5-(chloromethyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate |
SDF | Download SDF |
Canonical SMILES | O=C1OC2(C3=CC=C(C=C3OC4=C2C=CC(OC(C)=O)=C4)OC(C)=O)C5=C1C=C(CCl)C=C5 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |