RNase Inhibitor, Murine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
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RNase Inhibitor，Murine是从大肠杆菌中分离的50 kD重组蛋白，它最初来自小鼠的核糖核酸酶抑制蛋白基因。我们的产品RNase inhibitor对RNases A、B和C具有特异性，但不能抑制RNase 1、RNase T1、S1核酸酶、RNase H或自曲霉属的RNase。它能以1:1的比例非共价结合多种RNase，具有高亲和力。特别是，根据以前的研究，当与AMV或M-MuLV逆转录酶、taq DNA聚合酶或SP6/T7/T3 RNA聚合酶同时使用时，未观察到RNase Inhibitor对酶活性的抑制，这意味着它可以在各种实验中用于防止RNA降解。
与人源的RNase inhibitor相比，来自小鼠的RNase inhibitor缺乏对氧化非常敏感并导致抑制剂失活的一对半胱氨酸。因此，与来自人/猪的RNase inhibitor相比，鼠源RNase inhibitor显著改善其抗氧化性。并且它在低浓度DTT（小于1 mM）下稳定，该特征使其在低浓度DTT的反应中更为合适（例如Real-time RT-PCR）。
我们的产品可用于多种反应以防止RNA降解，例如RT-PCR、cDNA合成、体外转录，酶促RNA标记反应或任何其他应用。
该产品特异性地抑制RNases A，B和C
与来源于人/猪的RNase inhibitor相比，提高了抗氧化性
适合于需要低浓度DTT的反应（例如，Real-time RT-PCR）
| Size | |
| RNase Inhibitor, Murine(40 U/uL) | 3000 U (75 uL) |
| RNase Inhibitor, Murine(40 U/uL) | 15000 U (375 uL) |
所有组分储存于-20°C。 | |
1）Q：活性单位定义？
A：抑制5 ng RNase A活性的50%所需要的酶量定义为1个活性单位(U)。RNase A 的活性通过水解Cyclic 2’, 3’-CMP生成3’-CMP定量求得。
2）Q：与人源的RNase inhibitor相比，RNase Inhibitor, Murine有什么特点？
A：与人源RNase inhibitor相比，鼠源RNase inhibitor不含人源蛋白中的两个对氧化非常敏感的半胱氨酸，因而具有更高的抗氧化活性，且更加适合于对高DTT敏感的实验(如qPCR)。
3）Q：RNase Inhibitor, Murine常用浓度？
A：对于cDNA合成、体外转录、体外翻译等常见的反应体系中，为保护其中的RNA不被RNase降解，RNase Inhibitor, Murine推荐用量是最终浓度为1-2 U/μL。
4）Q：RNase Inhibitor, Murine能否抑制各种RNase？
A：我们的产品RNase inhibitor, Murine能够广泛抑制RNases A、B和C，但不能抑制RNase 1、RNase T1、S1核酸酶、RNase H或自曲霉属的RNase。
5）Q：RNase Inhibitor, Murine抑制RNase活性的最适pH值？
A：RNase inhibitor, Murine抑制RNase活性的pH值范围较广，在pH 7-8时表现最大活性。
6）Q：RNase Inhibitor, Murine保存条件？
A：产品-20℃保存，有效期2年。