ARCA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
mRNA加工中的关键步骤是添加5'端帽子结构,即在RNA的5'起始端和鸟苷酸之间的5'-5'三磷酸键,然后在鸟苷酸的N-7位点酶促甲基化以形成成熟的mCAP。
现在, 抗反向帽类似物(ARCA)被引入。ARCA只能以正确的方向插入,所形成的mRNA被翻译时的效率相当于mCAP起始的mRNA的两倍。
在制备合成mRNA时,通常在使用之前加帽,用以稳定mRNA并显著增强翻译。在转录反应中,使用cap类似物与GTP的4:1混合物将得到约80%的加帽mRNA。参考文献:
[1]. Ramanathan A1, Robb GB1, et al, mRNA capping: biological functions and applications. Nucleic Acids Res. 2016 Sep 19;44(16):7511-26.
[2]. Guo XR1,2, Wang XL2, et al, PDX-1 mRNA-induced reprogramming of mouse pancreas-derived mesenchymal stem cells into insulin-producing cells in vitro. Clin Exp Med. 2015 Nov;15(4):501-9.
[3]. Lee K1, Yu P2,et al, Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells. Int J Nanomedicine. 2015 Mar 6;10:1841-54.
[4]. Kenney SP1, Meng XJ2. The lysine residues within the human ribosomal protein S17 sequence naturally inserted into the viral nonstructural protein of a unique strain of hepatitis E virus are important for enhanced virus replication. J Virol. 2015 Apr;89(7):3793-803.
[5]. Almåsbak H1, Walseng E2,et al, Inclusion of an IgG1-Fc spacer abrogates efficacy of CD19 CAR T cells in a xenograft mouse model. Gene Ther. 2015 May;22(5):391-403.
- 1. Mingzhu Gao, Maoping Tang, et al. "Modulating Plaque Inflammation via Targeted mRNA Nanoparticles for the Treatment of Atherosclerosis." ACS Nano. 2023 Sep 26;17(18):17721-17739. PMID: 37669404
- 2. Jian Xu, Zhihua Yang, et al. "Rapid differentiation of hiPSCs into functional oligodendrocytes using an OLIG2 synthetic modified messenger RNA." Commun Biol. 2022 Oct 14;5(1):1095. PMID: 36241911
- 3. Jingjing Zhang, Yun Li, et al. "Genome-wide CRISPR/Cas9 library screen identifes PCMT1 as a critical driver of ovarian cancer metastasis." J Exp Clin Cancer Res. PMID: 35033172
Physical Appearance | Solution |
Storage | Store at -20°C or below |
M.Wt | 817.4 (free acid) |
Formula | C22H32N10O18P3 (free acid) |
Synonyms | Anti Reverse Cap Analog, 3´-O-Me-m7G(5')ppp(5')G |
SDF | Download SDF |
Canonical SMILES | OC1C(COP([O-])(OP([O-])(OP([O-])(OCC2OC(N3C=[N+](C)C4=C3N=C(N)NC4=O)C(O)C2OC)=O)=O)=O)OC(N5C=NC6=C5N=C(N)NC6=O)C1O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |