Protease Inhibitor Cocktail (100X in DMSO, EDTA plus)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Liu L, Yin S, Gan W. "TRAF6 Promotes PRMT5 Activity in a Ubiquitination-Dependent Manner." Cancers, 2023, 15(9): 2501. PMID: 37173967
- 2. Yu Z, Wu Z, et al. "Structural basis of a two-step tRNA recognition mechanism for plastid glycyl-tRNA synthetase." Nucleic Acids Research, 2023, 51(8): 4000-4011. PMID: 36912079
- 3. Reham Alsaadi. "Characterization of post-translational modification of ATG16L1 in antibacterial autophagy." University of Ottawa. 2019.
该产品是一种在细胞裂解和蛋白质提取过程中使用的蛋白酶抑制剂混合物(100X in DMSO, EDTA plus)，它是一种即用型混合浓缩原液，可用于抑制多种内源性蛋白酶。并含有单独的0.5 M EDTA溶液，可抑制金属蛋白酶。
它拥有六种最佳浓度的广谱蛋白酶抑制剂，溶解在 DMSO中，能够有效抑制细胞裂解物样品中出现的丝氨酸蛋白酶，半胱氨酸蛋白酶，天冬氨酸 - 蛋白酶和氨肽酶。 EDTA提供对金属蛋白酶的抑制。
EDTA通过螯合金属蛋白酶活性所必需的二价阳离子来抑制酶的活性，因此EDTA可能影响其它蛋白质的活性。在特定实验中可能需要预实验来确定EDTA是否会产生不良影响。 如果要使用固定化金属螯合亲和层析（IMAC）纯化目的蛋白或需要进行2D凝胶电泳分析，则必须在纯化前通过大量透析或脱盐除去EDTA。
使用前，在室温下解冻，然后在实验前以1:100 (v / v) 稀释至溶液样品(如细胞裂解物或组织提取物)。
应用：WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay等。
| Tube | Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A (100X in DMSO) | ||||||
| A2573 | AEBSF.HCl | 丝氨酸蛋白酶抑制剂 | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl | |
| A2574 | Aprotinin | 牛胰蛋白酶抑制剂 | Proteases|Serine Protease | 9087-70-1 | ||
| A2575 | Bestatin | 氨肽酶抑制剂 | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O | |
| A2576 | E-64 | 半胱氨酸蛋白酶（cysteine protease）抑制剂 | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O | |
| A2570 | Leupeptin | 丝氨酸和半胱氨酸蛋白酶抑制剂 | Proteases|Serine Protease | 103476-89-7 | O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] | |
| A2571 | Pepstatin A | 天冬氨酸蛋白酶抑制剂 | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O | |
| B (100X in ddH2O) | ||||||
| EDTA, disodium salt, dihydrate | 金属蛋白酶抑制剂 | protease | 6381-92-6 | |||
| 储存在-20°C，稳定保持至少12个月。 | ||||||