Nile Red
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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
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Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Nile Red, is a fluorescent stain specific for the detection of intracellular lipid droplets in hydrophobic environment, but show minimal fluorescence in aqueous media. Nile Red can be applied for staining intracellular lipids, lysosomal phospholipid inclusions and hydrophobic domains of proteins, and detected by fluorescence microscopy and flow cytofluorometry. In all organic solvents, Nile Red is intensely fluorescent, with colors ranging from golden yellow to red.
Reference:
1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973.
| Storage | Store at -20°C |
| M.Wt | 318.37 |
| Cas No. | 7385-67-3 |
| Formula | C20H18N2O2 |
| Solubility | ≥2.56 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | 9-(diethylamino)-5H-benzo[a]phenoxazin-5-one |
| SDF | Download SDF |
| Canonical SMILES | CCN(C1=CC2=C(N=C3C4=CC=CC=C4C(C=C3O2)=O)C=C1)CC |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
Monkey aortic smooth muscle cells and mouse peritoneal macrophages, induced by acetylated low density lipoprotein |
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Reaction Conditions |
100 ng/ml Nile Red |
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Applications |
Better selectivity for cytoplasmic lipid droplets was obtained when the cells were viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm). |
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Note |
The technical data provided above is for reference only. |
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References: 1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973. |
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质量控制和MSDS
- 批次:
化学结构
