EZ Cap™ Reagent AG
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EZ Cap Reagent AG是一种共转录加帽试剂，用于5'加帽mRNA的体外转录，产生Cap 1结构。Cap AG在文献中已经显示提供高达98％的加帽效率。
EZ Cap AG需要一个AG起始序列。EZ Cap AG通常提供> 90％的具有天然存在的Cap 1结构的加帽产物。
EZ Cap与野生型T7聚合酶兼容。建议使用5 mM EZ Cap和rNTPs。
模板设计是任何转录的重要组成部分。EZ Cap AG应与特定的起始序列5'AG 3'一起使用。
最后的cap效率取决于EZ Cap Reagent、DNA模板和最终的mRNA序列。由于RNA长度和碱基组成而形成的二级结构可能影响最终的加帽效率。
- 1. Yu Zhang, Xiaodong Xi, et al. "Chemically modified in-vitro-transcribed mRNA encoding thrombopoietin stimulates thrombopoiesis in mice." Mol Ther Nucleic Acids. 2022 Aug 13;29:657-671. PMID: 36090760
- 2. Binfan Chen, Yuchen Chen, et al. "A Single Dose of Anti-HBsAg Antibody-Encoding mRNA-LNPs Suppressed HBsAg Expression: a Potential Cure of Chronic Hepatitis B Virus Infection." mBio. 2022 Aug 30;13(4):e0161222. PMID: 35862767
- 3. Shudan Tan, Xue Hu, et al. "Preclinical evaluation of RQ3013, a broad-spectrum mRNA vaccine against SARS-CoV-2 variants." biorxiv.
- 4. Jing Lu, Guoliang Lu, et al. "A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice." Cell Res. 2020;1-4. PMID: 32801356
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 1145.60 (free acid) |
| Formula | C32H43N15O24P4 (free acid) |
| Synonyms | EZ Cap™ Reagent AG for co-transcriptional capping of mRNA,m7G(5')ppp(5')(2'OMeA)pG |
| SDF | Download SDF |
| Canonical SMILES | O=C1NC(N)=NC2=C1[N+](C)=CN2[C@@H]3[C@@H](O)[C@@H](O)[C@H](COP([O-])(OP([O-])(OP([O-])(OC[C@H]4O[C@@H](N5C=NC6=C5N=CN=C6N)[C@H](OC)[C@@H]4OP([O-])(OC[C@H]7O[C@@H](N8C=NC9=C8N=C(N)NC9=O)[C@H](O)[C@@H]7O)=O)=O)=O)=O)O3.[Na+].[Na+].[Na+] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
相关生物数据
