mCAP
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
真核生物和病毒的mRNA在5'起始端有一个单甲基化修饰的帽子结构,m7G(5')ppp(5')G或称为mCAP。 Cap类似物在多种细胞过程中起重要作用,比如蛋白合成的起始,避免核酸外切酶切割RNA,作为招募前体mRNA剪接、聚腺苷酸化和核输出所需蛋白因子的独特地识别信号。Cap可以与不同的结合蛋白、起始因子和核糖体元件相互作用。
mCAP是分子生物学工具中的第一个cap类似物。 mCAP以50%的机会以正确的方向插入,来增强翻译。 另外50%的分子不能成为有效翻译的底物,降低了转录本的特异活性。
通过将cap类似物和GTP的混合物(通常比例为4:1)可将mCAP掺入转录本中。大约80%的合成mRNA将具有5' cap,而其余的20%只具有5'三磷酸。
参考文献:
[1].Abbas YM1, Laudenbach BT2, et al, Structure of human IFIT1 with capped RNA reveals adaptable mRNA binding and mechanisms for sensing N1 and N2 ribose 2'-O methylations. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2106-E2115.
[2].Katibah GE1, Qin Y2, et al, Broad and adaptable RNA structure recognition by the human interferon-induced tetratricopeptide repeat protein IFIT5. Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12025-30.
[3]. Kuge H1, Brownlee GG, et al, Cap ribose methylation of c-mos mRNA stimulates translation and oocyte maturation in Xenopus laevis. Nucleic Acids Res. 1998 Jul 1;26(13):3208-14.
[4]. Ramanathan A1, Robb GB1, et al, mRNA capping: biological functions and applications. Nucleic Acids Res. 2016 Sep 19;44(16):7511-26.
[5]. Probst J1, Brechtel S, et al, Characterization of the ribonuclease activity on the skin surface. Genet Vaccines Ther. 2006 May 29;4:4.
[6]. Karikó K1, Buckstein M, et al, Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA. Immunity. 2005 Aug;23(2):165-75.
Physical Appearance | Solution |
Storage | Store at -20°C or below |
M.Wt | 803.4 (free acid) |
Formula | C21H30N10O18P3 (free acid) |
Synonyms | m7G(5')ppp(5')G Cap Analog, N7-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine |
SDF | Download SDF |
Canonical SMILES | OC1C(COP([O-])(OP([O-])(OP([O-])(OCC2OC(N3C=[N+](C)C4=C3N=C(N)NC4=O)C(O)C2O)=O)=O)=O)OC(N5C=NC6=C5N=C(N)NC6=O)C1O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |