Cyanine 3-dCTP
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cy3-dCTP可用于通过Taq聚合酶进行PCR,通过DNAse I / DNA聚合酶I进行Nick Translation,cDNA标记和3'末端标记直接酶标记DNA/cDNA。Cy3-dCTP可替代反应中的CTP,作为T4和Taq DNA聚合酶、大肠杆菌DNA聚合酶(全酶和Klenow片段)、逆转录酶(来自AMV和M-MuLV)和末端转移酶的底物。合成的染料标记的DNA/cDNA探针可用于通过原位杂交,微阵列或印迹技术鉴定特定序列。 Cy3-dCTP也可用于多色荧光标记。与尿苷的C5位连接的优化接头确保了优化的底物特性和标记效率。 推荐用于PCR和Nick Translation的Cy3-dCTP/dCTP比率:30-50%Cy3-dCTP/50%dCTP。
Physical Appearance | Solution |
Storage | Store at -20°C or below |
M.Wt | 1131.9 (free acid) |
Formula | C43H54N6O20P3S2 (free acid) |
Synonyms | Cyanine 3-5-Propargylamino-2'-deoxycytidine-5'-Triphosphate,Cy3-dCTP |
Chemical Name | tetralithium mono(2-((1E,3E)-3-(1-(6-((3-(4-amino-1-(4-hydroxy-5-(((oxido((oxido(phosphonatooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2-oxo-1,2-dihydropyrimidin-5-yl)prop-2-yn-1-yl)amino)-6-oxohexyl)-3,3-dimethyl-6-sulfonatoindolin-2- |
SDF | Download SDF |
Canonical SMILES | NC(C(C#CCNC(CCCCCN1C2=C(C=CC(S(=O)([O-])=O)=C2)C(C)(C)/C1=C\C=C\C3=[N+](CC)C(C=C(S(=O)([O-])=O)C=C4)=C4C3(C)C)=O)=CN5C6CC(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O6)=NC5=O.[Li+].[Li+].[Li+].[Li+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |