Ac-DEVD-AFC
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ac-DEVD-AFC是活化的caspase-3的荧光底物。Caspase-3切割D和AFC之间的四肽，从而释放出荧光AFC [1]。Caspase活性可以通过游离AFC的荧光检测来定量，该游离AFC在400 nm处激发并在505 nm处发射。注意是活化的caspase-3而非pro-caspase-3切割Ac-DEVD-AFC底物[2]。
References:
[1] Julien O, Wells JA. Caspases and their substrates. Cell Death Differ. 2017;24(8):1380–1389.
[2] Angelastro JM, Moon NY, Liu DX, et al. Characterization of a novel isoform of caspase-9 that inhibits apoptosis. J Biol Chem. 2001;276(15):12190–12200.
| Storage | Store at -20°C |
| M.Wt | 729.6 |
| Cas No. | 201608-14-2 |
| Formula | C30H34F3N5O13 |
| Synonyms | N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Trifluoromethylcoumarin,Caspase-3 Substrate (Fluorogenic) |
| Solubility | insoluble in H2O; ≥73 mg/mL in DMSO |
| Chemical Name | N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine |
| SDF | Download SDF |
| Canonical SMILES | CC(N[C@@H](CC(O)=O)C(N[C@H](C(N[C@@H](C(C)C)C(N[C@@H](CC(O)=O)C(NC1=CC(OC(C=C2C(F)(F)F)=O)=C2C=C1)=O)=O)=O)CCC(O)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
