Difopein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Difopein is an inhibitor of 14-3-3 protein.
14-3-3 protein has been involved in a wide range of biologic processes such as signal transduction, vesicular transport, cell cycle control, DNA replication, DNA repair and apoptosis. 14-3-3 protein has been associated with tumor genesis. 14-3-3 protein correlates with reduced tumor cell apoptosis and abbreviated patient survival. Up-regulation of 14-3-3 protein provides a survival advantage to tumor cells. Inhibition of 14-3-3 protein might trigger apoptosis, inhibit growth of established tumors, suppress de novo tumor formation, and promise a novel therapeutic approach for glioma treatment [1].
In human glioma U251 and U87 cells, difopein resulted in apoptosis characteristics, such as nuclear fragmentation, DNA laddering fragment and appearance of membrane-enclosed apoptotic bodies. Difopein induced apoptosis in a time-dependent manner [1]. Difopein induced glioma cell apoptosis through down-regulating Bcl-2, up-regulating Bax and activating caspase-9 and caspase-3. In vivo, difopein effectively hindered proliferation and triggered apoptosis of tumor cells implanted into nude mice [1].
Reference:
[1] Cao W, Yang X, Zhou J, et al. Targeting 14-3-3 protein, difopein induces apoptosis of human glioma cells and suppresses tumor growth in mice[J]. Apoptosis, 2010, 15(2): 230-241.
Physical Appearance | White lyophilised solid |
Storage | Store at -20°C |
M.Wt | 6387.17 |
Cas No. | 396834-58-5 |
Formula | C273H424N76O89S6 |
Solubility | Soluble to 5 mg/ml in H2O |
SDF | Download SDF |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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