NHS-SS-Biotin Kit

The NHS-SS-Biotin Kit contains reagents sufficient for 10 biotin labeling reactions. It is a water-soluble biotin reagent for labeling protein, antibody or other molecules with amidogen (NH2-). The biotin in the kit is sufficient and has been activated for direct use. The whole procedure is simple and quick.

• Amine-reaction—Reacting with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides.
• Labeling antibody—This kit can label antibodies to facilitate immobilization, purification or detection.
• Labeling protein—This kit can label proteins to facilitate immobilization, purification or detection.
• Labeling Cell surface molecule—This kit can label the cell surface proteins because the negatively charged reagent does not permeate cell membranes.
• Reversible-Disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule.
• Medium length—Spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain
• Solubility increased—Sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds.

NHS-SS-biotin (Succinimidyl-2-(biotinamido)-ethyl-1,3-dithiopropionate) is a long-chain cleavable amine-reactive biotinylation reagent that has a spacer arm of 24.3 A in length due to the existence of a cross-bridge within its chemical structure. The long spacer arm creates an increased length between a covalently modified molecule and the bicyclic biotin rings that greatly enhances the ability of the molecule to bind to avidin or streptavidin probes. The NHS ester group of NHS-SS-biotin reacts with the amine groups of proteins and other molecule forming an amide bond and releasing of sulfo-NHS. NHS-SS-Biotin is a water-insoluble molecule that requires the dissolution of organic solvents prior to be added to aqueous reactions.

1）Q：货号K1001-K1006的生物素化试剂盒中，生物素产品与哪些基团反应进行生物素化？

A：该系列试剂盒中生物素试剂（如Sulfo-NHS-LC-Biotin）上的NHS酯与伯胺反应。在蛋白质和多肽中，伯胺基团一般存在于赖氨酸残基的氨基末端和ε胺（侧链）上。

2）Q：带有磺酸基（Sulfo-）的Biotin与不带的Biotin有什么不同？

A：磺酸基（Sulfo-）Biotin可增加水溶性，常用于细胞表面标记；non-sulfonated Biotin需用DMSO或DMF等有机溶剂溶解，可透过细胞膜，常用于标记细胞内带有伯胺（NH2-）的蛋白。

3）Q：K1001-K1006试剂盒中Biotin产品有什么不同？

A：第一，带磺酸基（Sulfo-）的Biotin与不带Sulfo-的Biotin膜渗透性、水溶性不同；第二，(Sulfo) -NHS-Biotin试剂是一种短链生物素化试剂， (Sulfo) -NHS-LC-Biotin试剂是一种中等长度的生物素化试剂，(Sulfo) -NHS-SS-Biotin是一种间隔臂包含可裂解的二硫键的生物素化试剂。

4）HABA分析常见问题