C-6 NBD Ceramide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
C-6 NBD Ceramide (C-6 NBD神经酰胺) 是短链膜通透性神经酰胺的荧光类似物,可渗透细胞膜,可用作活细胞和固定细胞中高尔基体的选择性染色剂。C-6 NBD神经酰胺也可以标记线粒体和内质网,但是在孵育45分钟后,这些结构发出的荧光与背景没有区别[1]。C-6 NBD神经酰胺可用于研究鞘脂转运和代谢机制。该染料在水性溶剂中的荧光较弱,但在非质子溶剂和其他非极性环境中荧光会增加。激发/发射最大值约为466/536 nm。
用高尔基探针C6- NBD神经酰胺与钙指示剂fluo-3对细胞进行染色,可以对高尔基体和游离钙进行共定位[2]。C6- NBD神经酰胺可以用来研究弓形虫与宿主细胞相互作用期间的脂质运输[3]。
References:
[1] Lipsky NG, Pagano RE. A vital stain for the Golgi apparatus. Science. 1985 May 10;228(4700):745-7.
[2] Xue, S., Nicoud, M., Cui, J. et al. High concentration of calcium ions in Golgi apparatus. Cell Res 4, 97–108 (1994).
[3] de Melo EJ, de Souza W. Pathway of C6-NBD-Ceramide on the host cell infected with Toxoplasma gondii. Cell Struct Funct. 1996 Feb;21(1):47-52.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 575.74 |
Cas No. | 94885-02-6 |
Formula | N-((2S,3R,E)-1,3-dihydroxyoctadec-4-en-2-yl)-6-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)hexanamide |
Solubility | ≤5mg/ml in ethanol;10mg/ml in DMSO;25mg/ml in dimethyl formamide |
Chemical Name | CCCCCCCCCCCCC/C=C/[C@H]([C@H](CO)NC(CCCCCN([H])C1=CC=C(C2=NON=C12)[N+]([O-])=O)=O)O |
SDF | Download SDF |
Canonical SMILES | C30H49N5O6 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |