ARCA Cy3 EGFP mRNA (5-moUTP)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ARCA Cy3 EGFP mRNA (5-moUTP) can be used to analyze mRNA delivery and translation efficiency. The EGFP mRNA will express an enhanced version of green fluorescent protein, which was originally isolated from the jellyfish, Aequorea victoria. EGFP is a direct detection reporter gene commonly used in mammalian cell cultures to produce bright green fluorescence with an emission peak at 509 nm. EGFP mRNA labeled with cyanine 5 can be directly visualized. ARCA Cy3 EGFP mRNA (5-moUTP) is an ideal molecule for determining mRNA delivery and localization and is independent of translation.
Cyanine 3 is a synthetic fluorescent dye with maximum excitation and emission wavelengths of 550 nm and 570 nm, respectively. APExBIO ARCA Cy3 EGFP mRNA (5-moUTP) is transcribed in a ratio of 1:3 with Cyanine 3-UTP: 5-methoxy-UTP. Substitution in this ratio results in mRNA that is easy to visualize and can still be translated in cell culture. Translation efficiency is the opposite of Cyanine 3-UTP replacement.
ARCA Cy3 EGFP mRNA (5-moUTP) is restricted using APExBIO's proprietary co-transcriptional capping method to produce a naturally occurring Cap 0 structure with high end capping efficiency. It is polyadenylation, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics the fully processed mature mRNA.
|mRNA Length||996 nucleotides|
|Buffer||1 mM Sodium Citrate, pH 6.4||Storage||-40°C or below|
|General tips||请将其于冰上溶解,并小心防止RNase污染降解。尽可能避免反复冻融。 不要涡旋震荡。首次使用时，将其轻柔离心并分成几份，可供单独使用。使用不含RNase的试剂和耗材，使用适当的无RNase技术。直至与转染试剂混合，才可加入含有血清的培养基中。|