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ARCA Cy3 EGFP mRNA (5-moUTP)

Catalog No.
R1008
具备更好性能的5-moUTP修饰mRNA,带有Cy3标记,抑制RNA介导的先天免疫激活,具有稳定高效的表达效率,可进行定位以及实验对照。
组合的产品项目
规格价格库存 数量
100ug (1mg/mL)
¥ 4,620.00
现货
1mg (1mg/mL)
¥ 23,100.00
现货
5x1mg (1mg/mL)
¥ 85,800.00
现货

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Background

ARCA Cy3 EGFP mRNA (5-moUTP) can be used to analyze mRNA delivery and translation efficiency. The EGFP mRNA will express an enhanced version of green fluorescent protein, which was originally isolated from the jellyfish, Aequorea victoria. EGFP is a direct detection reporter gene commonly used in mammalian cell cultures to produce bright green fluorescence with an emission peak at 509 nm. EGFP mRNA labeled with cyanine 5 can be directly visualized. ARCA Cy3 EGFP mRNA (5-moUTP) is an ideal molecule for determining mRNA delivery and localization and is independent of translation.

Cyanine 3 is a synthetic fluorescent dye with maximum excitation and emission wavelengths of 550 nm and 570 nm, respectively. APExBIO ARCA Cy3 EGFP mRNA (5-moUTP) is transcribed in a ratio of 1:3 with Cyanine 3-UTP: 5-methoxy-UTP. Substitution in this ratio results in mRNA that is easy to visualize and can still be translated in cell culture. Translation efficiency is the opposite of Cyanine 3-UTP replacement.

ARCA Cy3 EGFP mRNA (5-moUTP) is restricted using APExBIO's proprietary co-transcriptional capping method to produce a naturally occurring Cap 0 structure with high end capping efficiency. It is polyadenylation, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics the fully processed mature mRNA.

Description

mRNA Length 996 nucleotides
Concentration1.0 mg/mL
Buffer 1 mM Sodium Citrate, pH 6.4 Storage -40°C or below
General tips请将其于冰上溶解,并小心防止RNase污染降解。尽可能避免反复冻融。 不要涡旋震荡。首次使用时,将其轻柔离心并分成几份,可供单独使用。使用不含RNase的试剂和耗材,使用适当的无RNase技术。直至与转染试剂混合,才可加入含有血清的培养基中。
Shipping Condition试用装:蓝冰运输。