2',7'-Dichlorofluorescein diacetate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
2',7'-Dichlorofluorescein diacetate (2′,7′-二氯荧光素二乙酸酯),也称为DCFDA或H2DCFDA,是一种具有细胞渗透性的荧光探针,可用于检测活性氧(ROS)和一氧化氮(·NO)以及确定总氧化应激程度[1]。DCFDA本是无荧光的,DCFDA进入细胞后被酯酶迅速脱酯化,在存在ROS(主要为H2O2)的情况下,被氧化转化为具有强绿色荧光的2',7'-dichlorofluorescein(2′,7′-二氯荧光素,DCF或H2DCF),激发/发射光谱为492/515 nm。荧光信号可以用荧光显微镜或流式细胞仪检测。
DCFDA可以应用于癌症研究,测定癌细胞内ROS的水平,例如乳腺癌[2],肝癌[3]。
References:
[1] Eruslanov E, Kusmartsev S. Identification of ROS using oxidized DCFDA and flow-cytometry. Methods Mol Biol. 2010;594:57-72.
[2] Figueroa D, Asaduzzaman M, Young F. Real time monitoring and quantification of reactive oxygen species in breast cancer cell line MCF-7 by 2',7'-dichlorofluorescin diacetate (DCFDA) assay. J Pharmacol Toxicol Methods. 2018 Nov - Dec;94(Pt 1):26-33.
[3] Ding M, Jiao G, Shi H, et al. Investigations on in vitro anti-carcinogenic potential of L-carnosine in liver cancer cells. Cytotechnology. 2018 Feb;70(1):163-167.
Storage | Store at -20°C |
M.Wt | 485.27 |
Cas No. | 2044-85-1 |
Formula | C24H14Cl2O7 |
Solubility | insoluble in EtOH; insoluble in H2O; ≥16.17 mg/mL in DMSO |
Chemical Name | 2',7'-dichloro-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate |
SDF | Download SDF |
Canonical SMILES | CC(OC1=C(Cl)C=C(C2(C(C=CC=C3)=C3C(O2)=O)C(C=C(Cl)C(OC(C)=O)=C4)=C4O5)C5=C1)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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