2,7-Dichlorodihydrofluorescein diacetate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
2,7-Dichlorodihydrofluorescein diacetate ，中文名称为2,7-二氯二氢荧光素二乙酸酯，也称为为H2DCFDA或DCFH-DA。DCFH-DA是一种细胞膜渗透性的活性氧（ROS）检测探针。DCFH-DA本身没有荧光，一旦进入细胞内后，可以被细胞内的酯酶脱酯化生成DCFH（2,7-二氯二氢荧光素），而DCFH不会透过细胞膜，因此积聚在细胞内。无荧光的DCFH被细胞内的活性氧氧化成有荧光的DCF（2,7-二氯荧光素）[1-2]。绿色荧光强度与活性氧的水平成正比。可使用共聚焦显微镜，荧光显微镜和流式细胞仪等仪器检测荧光信号。Ex/Em (nm)：485/527 [3]。
[1] Yazdani M. Concerns in the application of fluorescent probes DCDHF-DA, DHR 123 and DHE to measure reactive oxygen species in vitro. Toxicol In Vitro. 2015 Dec 25;30(1 Pt B):578-82.
[2]Kalyanaraman B, Darley-Usmar V, Davies KJ, et al. Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations. Free Radic Biol Med. 2012;52(1):1–6.
[3] Aranda A, Sequedo L, Tolosa L, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63.
| Storage | Store at -20°C |
| M.Wt | 487.29 |
| Cas No. | 4091-99-0 |
| Formula | C24H16Cl2O7 |
| Synonyms | H2DCFDA, DCFH-DA |
| Solubility | ≥48.7 mg/mL in DMSO; insoluble in H2O; ≥81.8 mg/mL in EtOH with gentle warming |
| Chemical Name | 2-(3,6-diacetoxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid |
| SDF | Download SDF |
| Canonical SMILES | CC(OC1=C(Cl)C=C(C(C2=CC(Cl)=C(OC(C)=O)C=C2O3)C4=CC=CC=C4C(O)=O)C3=C1)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
