ZM336372
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ZM336372是C-Raf的高效抑制剂，其IC50值为70 nM [1]。也有报道ZM336372能抑制SAPK2b/p38β2，其IC50值为2 μM。
C-Raf是丝氨酸/苏氨酸特异性蛋白激酶Raf激酶家族的成员，在ERK1/2信号通路中起重要作用。已报道C-Raf的异常表达与人类疾病相关，并且也参与一系列癌症。
ZM336372是高效的C-Raf抑制剂，与已报道的C-Raf抑制剂PD98059相比具有不同的选择性。在原代神经元中，ZM336372通过抑制C-Raf活化，从而降低低钾诱导的凋亡百分比[2]。H2O2和ZM336372预处理肿瘤球体24小时，通过介导ERK1/2信号通路，能完全消除ROS诱导的eNOS上调[3]。
参考文献：
1. Hall-Jackson, C.A., et al., Paradoxical activation of Raf by a novel Raf inhibitor. Chem Biol, 1999. 6(8): p. 559-68.
2. Burgess, S. and V. Echeverria, Raf inhibitors as therapeutic agents against neurodegenerative diseases. CNS Neurol Disord Drug Targets, 2010. 9(1): p. 120-7.
3. Wartenberg, M., et al., Reactive oxygen species-mediated regulation of eNOS and iNOS expression in multicellular prostate tumor spheroids. Int J Cancer, 2003. 104(3): p. 274-82.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 389.4 |
| Cas No. | 208260-29-1 |
| Formula | C23H23N3O3 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥19.45 mg/mL in DMSO |
| Chemical Name | 3-(dimethylamino)-N-[3-[(4-hydroxybenzoyl)amino]-4-methylphenyl]benzamide |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(C=C(C=C1)NC(=O)C2=CC(=CC=C2)N(C)C)NC(=O)C3=CC=C(C=C3)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | ZM336372是高效选择性c-Raf抑制剂，其IC50值为70 nM。 | |||||
| 靶点 | C-Raf | |||||
| IC50 | 70 nM | |||||
化学结构
