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ZIP (SCRAMBLED)

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Catalog No.
A8830
PKMζ抑制剂
组合的产品项目
规格价格库存 数量
1mg
¥ 930.00
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Background

IC50: By suppressing protein kinase Mξ (PKMξ), ZIP reverses late-phase LTP with an IC50 of 1 - 2.5 μM.

Long-term potentiation (LTP), a persistent synaptic enhancement, is considered to be a substrate for memory. A typical LTP process includes two phases, induction and maintenance. PKMξ as an active form of protein kinase C (PKC) isozyme is necessary and potent for LTP maintenance. ZIP is applied as a novel and cell-permeable inhibitor for PKMξ and can therefore block LTP. [1]

In vitro: In order to determine the specific phase of LTP affected by PKMξ, ZIP was added at different concentrations to the bath before cells incubation. Experiments with ZIP were compared with experiments without the peptide. This study showed that ZIP could selectively inhibit PKMξ-induced synaptic potentiation in hippocampal slices in vitro. [2]

In vivo: ZIP was the first tool available to test the maintenance hypothesis in vitro. The effect of ZIP on late phase of LTP in vivo was also detected using the rat hippocampus. It was found that intra-hippocampal injection of ZIP with a dosage of 10 nmol in 1 ml saline rapidly reversed the late-phase LTP and led to persistent loss of 1-day-old spatial memory. [3]

Clinical trial: So far, no clinical trial has been conducted.

References:
[1]Ling SF, Benardo LS, Serrano PA, Blace N, Kelly MT, Crary JF and Sacktor TC.  Protein kinase Mξ is necessary and sufficient for LTP maintenance. Nat. Neurosci. 2002 Apr. 5(4): 2956.
[2]Serrano P, Yao Y and Sacktor TC.  Persistent phosphorylation by protein kinase maintains late-phase long-term potentiation. J. Neurosci. 2005 Feb. 25(8): 1979–84.
[3]Pastalkova E, Serrano P, Pinkhasova D, Wallace E, Fenton AA, Sacktor TC.  Storage of spatial information by the maintenance mechanism of LTP. Science. 2006 Aug. 313: 1141-4.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt1928.4
Cas No.908012-18-0
FormulaC90H154N30O17
Synonymsz-Pseudosubstrate inhibitory peptide
Solubility≥192.8mg/mL in DMSO
SDFDownload SDF
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

质量控制

化学结构

ZIP