ZIP (SCRAMBLED)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50值:ZIP通过抑制蛋白激酶Mξ (PKMξ),逆转后期LTP,IC50值范围为1 - 2.5 μM。
长时程增强(LTP)指持续性突触增强,被当作记忆底物。一个典型的LTP过程包括两个阶段:诱导和维持。作为蛋白激酶C(PKC)同工酶的活性形式,PKMξ对于LTP的维持是必需且有效的。ZIP用作新型的、细胞可透过性的PKMξ抑制剂,因此可以阻止LTP[1]。
体外实验:为了确定受PKMξ影响的LTP的特定阶段,在细胞孵育前,将不同浓度的ZIP加入细胞培养基中。将ZIP与没有ZIP多肽的实验相比较。该研究表明,ZIP可以选择性地抑制体外海马切片中PKMξ诱发的突触增强[2]。
体内实验:ZIP是第一个可用于体外检测维持假设的工具。用大鼠海马也检测了ZIP对体内LTP晚期的影响。将溶于1 ml生理盐水的剂量为10 nmol的ZIP注射入海马内可迅速逆转晚期LTP,且导致一日龄动物空间记忆的持续损失[3]。
临床试验:到目前为止,还未进行临床试验。
参考文献:
[1]Ling SF, Benardo LS, Serrano PA, Blace N, Kelly MT, Crary JF and Sacktor TC. Protein kinase Mξ is necessary and sufficient for LTP maintenance. Nat. Neurosci. 2002 Apr. 5(4): 2956.
[2]Serrano P, Yao Y and Sacktor TC. Persistent phosphorylation by protein kinase maintains late-phase long-term potentiation. J. Neurosci. 2005 Feb. 25(8): 1979–84.
[3]Pastalkova E, Serrano P, Pinkhasova D, Wallace E, Fenton AA, Sacktor TC. Storage of spatial information by the maintenance mechanism of LTP. Science. 2006 Aug. 313: 1141-4.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 1928.4 |
Cas No. | 908012-18-0 |
Sequence | RLYRKRIWRSAGR |
Formula | C90H154N30O17 |
Synonyms | z-Pseudosubstrate inhibitory peptide |
Solubility | ≥192.8 mg/mL in DMSO; insoluble in EtOH; ≥2.18 mg/mL in H2O with gentle warming and ultrasonic |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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