Z-VDVAD-FMK
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Jurkat T淋巴细胞用一种不可逆的caspase-2抑制剂，benzyloxycarbonyl-Val-Asp(OMe)-
Val-Ala-Asp(OMe)-fluoromethyl ketone（Z-VDVAD-FMK）处理或稳定转染pro-caspase-2反义分子（Casp-2/AS）后可抵抗etoposide（依托泊苷）刺激的细胞色素c的释放[1]。当etoposide诱导的pro-caspase-2的激活被Z-VDVAD-FMK或稳定转染pro-caspase-2反义分子抑制时，细胞色素c的释放及其它细胞凋亡的表现均减弱。
在牛脑微血管内皮细胞中，OxyHb显著激活caspase-2和caspase-3。不可逆的caspase抑制剂Z-VDVAD-FMK（caspse-2抑制剂）和Z-DEVD-FMK（caspase-3抑制剂）显著减少细胞脱离、caspase-2和-3的活性、DNA梯度和PARP2的蛋白水解切割。在内皮细胞中，caspase-2和caspase-3的激活对OxyHb诱导的细胞凋亡是必需的，而Z-VDVAD-FMK和Z-DEVD-FMK具有保护细胞的潜力。
Caspase-2最小长度的抑制剂Z-VDVAD-FMK也抑制caspases 3和7[3]，阻止doxorubicin诱导的核凋亡，而非细胞死亡[4]。
参考文献：
1. J. D. Robertson, M. Enoksson et al. Caspase-2 Acts Upstream of Mitochondria to Promote Cytochrome c Release during Etoposide-induced Apoptosis. The Journal of Biological Chemistry. 277, :29803–29809, 2002
2. T. Meguro, B. Chen et al. Caspase Inhibitors Attenuate Oxyhemoglobin-Induced Apoptosis in Endothelial Cells, Stroke. 2001; 32:561-566.
3. Talanian, R. V., Quinlan, C., Trautz, S., Hackett, M. C., Mankovich, J. A., Banach, D., Ghayur, T., Brady, K. D., and Wong, W. W. (1997). Substrate specicity of caspase family proteases. J. Biol. Chem. 272, 9677–9682.
4. Gamen et al (2000) Doxorubicin treatment activates a Z-VAD-sensitive caspase, which causes Dym loss, caspase-9 activity, and apoptosis in Jurkat cells. Exp.Cell Res. 258 223.
| Storage | Store at -20°C |
| M.Wt | 695.73 |
| Cas No. | 210344-92-6 |
| Formula | C32H46N5O11F |
| Synonyms | Z-VDVAD-fluoromethylketone, Caspase-2 Inhibitor (fluoromethylketone),Z-Val-Asp(OMe)-Val-Ala-Asp(OMe)-FMK |
| Solubility | ≥34.8 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | methyl (3S)-5-fluoro-3-[[(2S)-2-[[(2S)-2-[[(2S)-4-methoxy-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]-4-oxobutanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]-4-oxopentanoate |
| SDF | Download SDF |
| Canonical SMILES | CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CC(=O)OC)NC(=O)C(C(C)C)NC(=O)OCC1=CC=CC=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1,2]: | |
|
细胞系 |
Jurkat T淋巴细胞 |
|
制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液，一般步骤如下：请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月。 |
|
反应条件 |
25 or 100 μM；1或22小时 |
|
实验结果 |
在Jurkat T淋巴细胞中，预先给予细胞25 μM Z-VDVAD-FMK并将其孵育1小时，或使用pro-caspase-2的反义链（Casp-2/AS）进行转染，均能抑制Etoposide诱导的细胞色素c释放。MTT测试结果表明，给予Jurkat细胞100 μM Z-VAD-FMK，孵育22小时，可以防止Doxorubicin诱导的核凋亡，但不能抑制细胞死亡。 |
|
References: [1]. J. D. Robertson, M. Enoksson et al. Caspase-2 Acts Upstream of Mitochondria to Promote Cytochrome c Release during Etoposide-induced Apoptosis. The Journal of Biological Chemistry. 277, :29803–29809, 2002. [2]. Gamen et al (2000) Doxorubicin treatment activates a Z-VAD-sensitive caspase, which causes Dym loss, caspase-9 activity, and apoptosis in Jurkat cells. Exp.Cell Res. 258 223. |
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| Z-VDVAD-FMK是一种不可逆的caspase-2抑制剂，在内皮细胞中衰减氧合血红蛋白诱导的PARP的切割和细胞凋亡。 | ||||||
| 靶点 | Caspase-2 | |||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
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