Z-DQMD-FMK
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
由Z-DQMD-FMK（Z-Asp(OMe)-Gln-Met-Asp(OMe)-fluoromethylketone）诱导的caspase-3抑制不可以恢复缺锌组的细胞数，但导致完整PKC-δ断裂为56 kDa的片段[1]。
特异性caspase抑制剂（Z-Asp(OMe)-Gln-Met-Asp(OMe)-fluoromethylketone）的抑制作用表明，在小细胞肺癌细胞中，caspase-8活化和线粒体损伤介导MG132诱导的细胞凋亡和GSH耗竭，最终激活caspase-9和caspase-3[2]。
旨在探讨中风后εPKC断裂与Caspase-3活化的因果关系，我们研究了细胞渗透性caspase-3特异性抑制剂Z-DQMD-FMK对断裂εPKC片段产生的作用。对于中风后的缺血中心区和缺血半暗带，Caspase-3抑制不能抑制完整PKC-δ和43 kDa片段的减少[3]。
参考文献：
1. Susan S. CHOU*, Michael S. CLEGG, Alterations in protein kinase C activity and processing during zinc-deficiency-induced cell death, Biochem. J. (2004) 383, 63–71 2. J. H. Banga, E. S. Han. Differential response of MG132 cytotoxicity against small cell lung cancer cells to changes in cellular GSH contents. Biochemical Pharmacology 68 (2004) 659–666. 3. T. Shimohata, H. Zhao, εPKC May Contribute to the Protective Effect of Hypothermia in a Rat Focal Cerebral Ischemia Model. Stroke. 2007;38:375-380
- 1.Wang J, Shao L, et al. "Synergetic Inhibition of Human Colorectal Cancer Cells by Combining Polyyne-Enriched Fraction from Oplopanax elatus and Irinotecan." Nutr Cancer. 2018 Oct 29:1-11. PMID:30372160
- 2.Han J, Yang BP, et al. "RhoB/ROCK mediates oxygen-glucose deprivation-stimulated syncytiotrophoblast microparticle shedding in preeclampsia." Cell Tissue Res. 2016 PMID:27324125
- 3.Li, Lingfei, et al. "P38/MAPK contributes to endothelial barrier dysfunction via MAP4 phosphorylation-dependent microtubule disassembly in inflammation-induced acute lung injury." Scientific reports 5 (2015). PMID:25746230
| Storage | Store at -20°C |
| M.Wt | 685.72 |
| Cas No. | 767287-99-0 |
| Formula | C29H40FN5O11S |
| Synonyms | Z-DQMD-FMK,Benzyloxycarbonyl-Asp(OMe)-Gln-Met-Asp(OMe)-fluoromethylketone |
| Solubility | ≥29.2 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | methyl (3S)-3-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-4-methoxy-4-oxo-2-(phenylmethoxycarbonylamino)butanoyl]amino]-5-oxopentanoyl]amino]-4-methylsulfanylbutanoyl]amino]-5-fluoro-4-oxopentanoate |
| SDF | Download SDF |
| Canonical SMILES | COC(=O)CC(C(=O)CF)NC(=O)C(CCSC)NC(=O)C(CCC(=O)N)NC(=O)C(CC(=O)OC)NC(=O)OCC1=CC=CC=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
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细胞系 |
3T3-Swiss白化病细胞 |
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制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液，一般步骤如下：请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。 |
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反应条件 |
25 μM；24小时 |
|
实验结果 |
在缺锌的3T3-Swiss白化病细胞中，Z-DQMD-FMK (25 μM)阻止caspase-3活化。Z-DQMD-FMK不能使细胞数目恢复，但促进全长PKC-δ加工成56-kDa片段。 |
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References: [1]. Susan S. CHOU*, Michael S. CLEGG, Alterations in protein kinase C activity and processing during zinc-deficiency-induced cell death, Biochem. J. (2004) 383, 63–71. |
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| Z-DQMD-FMK是Caspase-3的抑制剂，体外抑制MG132诱导的小细胞肺癌细胞死亡。 | ||||||
| 靶点 | Caspase-3 | |||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
相关生物数据
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