In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
XL228, a tyrosine kinase inhibitor, is involved in binding to and inhibiting the activities of multiple tyrosine kinases, such as the insulin-like growth factor 1 receptor (IGF1R), Src tyrosine kinase, and Bcr-Abl tyrosine kinase. Blockade of these kinases may result in the inhibition of tumor angiogenesis, cell proliferation, and metastasis . XL228 is a multitargeted protein kinase inhibitor targeting IGF1R, the aurora kinases, IGF-1R, cSrc, BCR/Abl and SRC kinases .
In vitro: XL228(5-100 nM) reduced cell survival by 10-70% in a dose and time dependent manner and inhibited migration and invasion of two tumors with high propensity to metastasize, FaDu and H460. Treatment with 50 and 100 nM XL228 abolished the ability of H460, A549 and FaDu cells to form colony. At 10 nM, XL228 significantly increased the radiosensitivity of H460, A549 and FaDu cells by enhancement factors (EF, at the survival fraction of 0.5) of 1.52, 1.31 and 1.67 respectively. But, In HN-5 cells, sensitization occurred only at 100 nM (EF = 2.27). In HN-5 cells, XL228 (100 nM)incubation induced accumulation of cells at the radiation sensitive G2/M phase of the cell cycle and induced apoptosis in 32% of cells .
Clinical trial: In a phase I study in patients with solid tumors or hematologic malignancies, XL228 showed a manageable toxicity profile and biological activity through decrease in target pathway and alternations in tumor nuclei .
 Smith D C, Britten C, Garon E B. A phase I study of XL228, a multitargeted protein kinase inhibitor, in patients with solid tumors or multiple myeloma[J]. J ClinOncol, 2010, 28(15s suppl: abstr 3105).
 Matsumoto F, Molkentine D, Clary D O, et al. A multi-kinase inhibitor, XL228, enhanced human cancer cell radiosensitivity and suppressed cell invasion and migration[J]. Cancer Research, 2011, 71(8 Supplement): 2487-2487.
 Smith D C, Britten C, Clary D O, et al. A phase I study of XL228, a potent IGF1R/AURORA/SRC inhibitor, in patients with solid tumors or hematologic malignancies[J]. J ClinOncol, 2009, 27(15 suppl): 149s.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥21.9 mg/mL in DMSO, ≥27.6 mg/mL in EtOH with ultrasonic and warming, <2.12 mg/mL in H2O|