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X-NeuNAc

现货
Catalog No.
A3944
神经氨酸酶活性显色试验的底物
组合的产品项目
规格价格库存 数量
10mg
¥ 700.00
Ship with 10-15 days
50mg
¥ 2,280.00
Ship with 10-15 days

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Background

Km: 0.89 mM for neuraminidase

X-Neu5Ac is a new substrate for chromogenic assay of neuraminidase activity in bacterial expression systems.

Many studies have focused on the functional role of cell surface gangliosides, which are thought to facilitate antigenicity, cell-cell recognition and cell growth regulation. Nacetyl neuraminic acid (Neu5Ac) within gangliosides Plays a key function in these roles. Chromogenic substrates, such as Neu5Ac, are used routinely for cytologic, histologic and spectroscopic analyses of enzyme activity.

In vitro: X-Neu5Ac was hydrolyzed by neuraminidase to release a halogenated product undergoing rapid aerobic oxidation to form the dark blue pigment. Preliminary kinetic studies indicated that X-Neu5Ac was a good and stable substrate for neuraminidase. In addition, X-Neu5Ac would also be hydrolyzed mutant enzymes [1].

In vivo: To visualize extracellular sialidase activity on the membrane surface in the rat brain, acute brain slices were incubated with X-Neu5Ac at pH 7.3. After 1h, myelin-abundant regions showed intense fluorescence in the rat brain. Although the hippocampus showed weak fluorescence in the brain, mossy fiber terminals in the hippocampus showed relatively intense fluorescence. In addition, the fluorescence intensities caused by X-Neu5Ac was correlated with the sialidase activity. Therefore, staining with X-Neu5Ac was specific for sialidase and useful for quantitative analysis of sialidase activities [1].

Clinical trial: N/A

References:
[1] Fujii I,Iwabuchi Y,Teshima T,Shiba T,Kikuchi M.  X-Neu5Ac: a novel substrate for chromogenic assay of neuraminidase activity in bacterial expression systems. Bioorg Med Chem.1993 Aug;1(2):147-9.
[2] Minami A,Shimizu H,Meguro Y,Shibata N,Kanazawa H,Ikeda K,Suzuki T.  Imaging of sialidase activity in rat brain sections by a highly sensitive fluorescent histochemical method. Neuroimage.2011 Sep 1;58(1):34-40.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt559.72
Cas No.160369-85-7
FormulaC19H21BrClN2NaO9
Solubility≥28mg/mL in DMSO
Chemical Namesodium;(2S,4S,6R)-5-acetamido-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-4-hydroxy-6-[(1S,2S)-1,2,3-trihydroxypropyl]oxane-2-carboxylate
SDFDownload SDF
Canonical SMILESCC(=O)NC1C(CC(OC1C(C(CO)O)O)(C(=O)[O-])OC2=CNC3=C2C(=C(C=C3)Br)Cl)O.[Na+]
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

质量控制

质量控制和MSDS

批次:

化学结构

X-NeuNAc