Windorphen
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Windorphen是一种Wnt抑制剂,其选择性地废除用于腹侧发育的Wnt信号传导.
Wnt蛋白是胚胎发育\细胞增殖\迁移和成人组织稳态期间在Wnt/β连环蛋白通路上相互作用的信号蛋白家族.
在典型Wnt/β连环蛋白报告细胞系STF293中,Windorphen剂量依赖性地抑制Wnt3a诱导的TOPFLASH萤光素酶活性(IC50为1.5 mM).在组成性激活Wnt信号传导的APC基因缺陷人类结肠腺癌SW480细胞中,72小时Windorphen治疗导致广泛的细胞凋亡.[1]
在斑马鱼胚胎中,Windorphen导致六体节阶段(krox0)背侧标记(pax2.1)和菱脑结3和5标记的明显扩张.Windorphen选择性抑制50％外包阶段胚胎(5.3 hpf)的腹侧和横向区域的Wnt信号传导.此外,Windoprhen治疗抢救mbl突变斑马鱼端脑/眼表型(即Axin1/β连环蛋白破坏复合体缺陷导致Wnt信号传导的异常激活以及端脑和眼的损失).[1]
参考文献：
1. Hao J, Ao A, Zhou L et al. Selective small molecule targeting β-catenin function discovered by in vivo chemical genetic screen. Cell Rep. 2013 Sep 12;4(5):898-904.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 302.75 |
| Cas No. | 19881-70-0 |
| Formula | C17H15ClO3 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥11.45 mg/mL in DMSO |
| Chemical Name | (E)-3-chloro-2,3-bis(4-methoxyphenyl)acrylaldehyde |
| SDF | Download SDF |
| Canonical SMILES | O=C/C(C1=CC=C(OC)C=C1)=C(Cl)\C2=CC=C(OC)C=C2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
