WIN 18446
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
WIN 18446 is a bis-(dichloroacetyl)-diamine which inhibits aldehyde dehydrogenase 1a2 (ALDH1a2), with IC50 value of 300 nM [1].
ALDH1a2, expressed almost exclusively in the testes, localizes to developing germ cells within the seminiferous tubules and strongly binds retinol, but does not recognize acetaldehyde as a substrate, suggesting that ALDH1a2 functions in testicular retinoic acid biosynthesis [1].
In whole testes or germ cells isolated from mice at 2 days postpartum, WIN 18,446 alone (1 μM) or in combination with retinol significantly reduced the expression of Stra8, a marker for retinoic acid activity [2].
In male rabbits, oral administration of WIN 18446 at 200 mg/kg for 16 weeks substantially reduced intratesticular concentrations of retinoic acid, significantly impaired spermatogenesis, and resulted in infertility. Reduced concentrations of intratesticular retinoic acid became obvious after only 4 weeks of treatment and preceded the decrease in sperm counts and the loss of mature germ cells in tissue samples. Sperm counts and fertility recovered after treatment was discontinued [1].
References:
[1]. Amory J K, Muller C H, Shimshoni J A, et al. Suppression of spermatogenesis by bisdichloroacetyldiamines is mediated by inhibition of testicular retinoic acid biosynthesis. Journal of Andrology, 2011, 32(1): 111-119.
[2]. Hogarth C A, Evanoff R, Snyder E, et al. Suppression of Stra8 expression in the mouse gonad by WIN 18,446. Biology of Reproduction, 2011, 84(5): 957-965.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 366.11 |
Cas No. | 1477-57-2 |
Formula | C12H20Cl4N2O2 |
Solubility | insoluble in H2O; ≥10.68 mg/mL in EtOH with ultrasonic; ≥11.4 mg/mL in DMSO |
Chemical Name | N,N'-(octane-1,8-diyl)bis(2,2-dichloroacetamide) |
SDF | Download SDF |
Canonical SMILES | ClC(C(NCCCCCCCCNC(C(Cl)Cl)=O)=O)Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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