Verapamil HCl
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Liying Chen, Zhi Chen, et al. "Study on mechanism of elemene reversing tumor multidrug resistance based on luminescence pharmacokinetics in tumor cells in vitro and in vivo." RSC Adv. 2020, 10, 34928-34937.
- 2. Li Y, Cao F, et al. "Hydroxychloroquine induced lung cancer suppression by enhancing chemo-sensitization and promoting the transition of M2-TAMs to M1-like macrophages." J Exp Clin Cancer Res. 2018 Oct 29;37(1):259. PMID:30373678
Storage | Store at -20°C |
M.Wt | 491.06 |
Cas No. | 152-11-4 |
Formula | C27H39ClN2O4 |
Solubility | ≥14.45 mg/mL in DMSO; ≥6.41 mg/mL in H2O with ultrasonic; ≥8.95 mg/mL in EtOH with ultrasonic |
Chemical Name | 2-(3,4-dimethoxyphenyl)-5-[2-(3,4-dimethoxyphenyl)ethyl-methylamino]-2-propan-2-ylpentanenitrile;hydrochloride |
SDF | Download SDF |
Canonical SMILES | CC(C)C(CCCN(C)CCC1=CC(=C(C=C1)OC)OC)(C#N)C2=CC(=C(C=C2)OC)OC.Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验[1]: | |
细胞系 |
骨髓瘤细胞系(JK-6L,RPMI8226和ARH-77细胞系) |
溶解方法 |
在DMSO中的溶解度>14.5mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
70 μM; 16 h |
应用 |
在骨髓瘤细胞系中,bortezomib(10nM)和verapamil(70μM)的组合显著降低了JK-6L,RPMI8226和ARH-77细胞系的活力。 JK-6L细胞对bortezomib和verapamil治疗更敏感。Bortezomib和verapamil的组合可能主要诱导凋亡性细胞死亡和caspase 3/7的活化。 |
动物实验[2]: | |
动物模型 |
胶原诱导的关节炎(CIA)小鼠模型 |
剂量 |
20 mg/kg; 腹腔注射,每天给药,从第21天开始 |
应用 |
在CIA小鼠模型中,verapamil显著减弱关节炎的发展和缓解炎症。Verapamil也显著降低炎症相关分子的mRNA水平,包括IL-1β,IL-6,NOS-2和COX-2。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同,这是由实验系统的误差引起的,属于正常现象。 |
References: [1]. Meister S1, Frey B, Lang VR, et al. Calcium channel blocker verapamil enhances endoplasmic reticulum stress and cell death induced by proteasome inhibition in myeloma cells. Neoplasia. 2010 Jul;12(7):550-61. [2]. Wang W1, Li Z2, Meng Q3, et al. Chronic Calcium Channel Inhibitor Verapamil Antagonizes TNF-α-Mediated Inflammatory Reaction and Protects Against Inflammatory Arthritis in Mice. Inflammation. 2016 Oct;39(5):1624-34. |
质量控制和MSDS
- 批次: