UPF 1069
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
UPF 1069是异喹啉酮的衍生物，是一种有效的聚(ADP-核糖)聚合酶2（PARP-2）的选择性抑制剂，50%抑制浓度IC50为0.3 μmol/L，在PARP-1-/-成纤维细胞的核提取物和重组酶制备中都可减少PAR形成。虽然其对PARP-2的选择性是所有PARP-2抑制剂中最强的，UPF 1069对PARP-1也有较弱的效力（IC50值为8 μmol/L）。UPF 1069被用于研究PARP-1和PARP-2在后缺血性脑损伤中的作用，发现其可在海马切片中加重缺氧缺糖（OGD）损伤。
参考文献：
Moroni F, Formentini L, Gerace E, Camaioni E, Pellegrini-Giampietro DE, Chiarugi A, Pellicciari R. Selective PARP-2 inhibitors increase apoptosis in hippocampal slices but protect cortical cells in models of post-ischaemic brain damage. Br J Pharmacol. 2009;157(5):854-862
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 279.29 |
| Cas No. | 1048371-03-4 |
| Formula | C17H13NO3 |
| Synonyms | UPF-1069,UPF1069 |
| Solubility | insoluble in H2O; ≥12.7 mg/mL in DMSO; ≥6.73 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | 5-phenacyloxy-2H-isoquinolin-1-one |
| SDF | Download SDF |
| Canonical SMILES | C1=CC=C(C=C1)C(=O)COC2=CC=CC3=C2C=CNC3=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| UPF 1069 is a selective inhibitor of PARP2 with IC50 of 0.3 μM. It is ~27-fold selective against PARP1. | ||||||
| Targets | PARP2 | |||||
| IC50 | 0.3 μM | |||||
化学结构
