UNC3866
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
UNC3866是一种强效的CBX4和CBX7 chromodomains拮抗剂，Kd值均为约100 nM，选择性是其他七种CBX和CDY chromodomains的6至18倍。UNC3866拮抗chromodomains的Polycomb CBX和CDY家族的甲基赖氨酸（KME）阅读功能。[1]
UNC3866是已报道的最强效的CBX7配体，Kd为 97±2.4 nM。通过AlphaScreen（IC50 = 66±1.2 nM）检测，UNC3866拮抗CBX7-H3的相互作用。UNC3866对CBX2、CBX4、CBX6和CBX8的亲和性与每个chromodomain和CBX7的序列同一性百分比紧密相关联。UNC3866对最类似于CBX7的CBX4同等强效，对CBX4和CBX7的选择性分别为CBX2、CBX6和CBX8的18、6和12倍以上。UNC3866对CBX4和CBX7的选择性超过CDY1的65倍，超过CDYL1b和CDYL2的选择性9倍。UNC3866每个骨架酰胺参与和CBX7骨架结合的至少一个氢键，而UNC3866的N-末端叔丁基苯甲酰帽主要连接D50、R52和L53的侧链。X射线晶体学显示，UNC3866和CBX chromodomains的相互作用密切模拟甲基化H3尾部的相互作用。UNC3866抑制PC3细胞增殖，与CBX7过表达以赋予生长优势的已知能力相一致。[1]
参考文献：
A cellular chemical probe targeting the chromodomains of Polycomb repressive complex 1. Nat Chem Biol. 2016 Mar;12(3):180-7.
| Storage | Store at -20°C |
| M.Wt | 795.02 |
| Cas No. | 1872382-47-2 |
| Formula | C43H66N6O8 |
| Solubility | Soluble in DMSO |
| Chemical Name | (3S,6S,9S,12S,15S)-methyl 3-benzyl-1-(4-(tert-butyl)phenyl)-12-(4-(diethylamino)butyl)-15-(hydroxymethyl)-9-isobutyl-6-methyl-1,4,7,10,13-pentaoxo-2,5,8,11,14-pentaazahexadecan-16-oate |
| SDF | Download SDF |
| Canonical SMILES | CC(C)(C)C1=CC=C(C(N[C@@H](CC2=CC=CC=C2)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](CCCCN(CC)CC)C(N[C@@H](CO)C(OC)=O)=O)=O)=O)=O)=O)C=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
相关生物数据
