UNBS 5162
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
UNBS5162是CXCL趋化因子的泛拮抗剂。通过MTT比色法实验,UNBS3157和UNBS5162对九个人类癌细胞系的平均抗增殖活性的IC50值分别为19.8和17.9 μ M。
CXCL趋化因子由一个CXC趋化因子结构域、一个粘蛋白样柄、一个跨膜结构域和一个含有潜在的可能与SH2结合的酪氨酸磷酸化位点的胞质尾区组成。
UNBS5162在体外具有弱的抗增殖活性。1 μM的UNBS5162不能显著改变PC-3或DU-145的细胞周期动力学。UNBS5162在1 μM下可引起Rb、pRb和E2F1蛋白表达的无标记修饰。浓度高于1 μM的UNBS5162有毒性,可抑制小鼠和人类造血干细胞及祖细胞的增殖。
在原位人PC-3前列腺癌模型中,UNBS5162可提高taxol的治疗效果。PC-3细胞和DU-145前列腺癌细胞的增殖在体外可被10 μM的UNBS5162在6天的治疗后抑制。流式细胞仪分析表明,PC-3和DU-145细胞使用10 μM的UNBS5162持续处理72 h可明显将PC-3细胞阻滞于G2期,在DU-145细胞中则程度较轻。处于G2/M期的PC-3细胞的百分比显著提高,处于G1期的细胞百分比则相应下降。使用10 μM的UNBS5162处理48和72小时,PC-3细胞中的Rb蛋白表达完全消失。
参考文献:
[1]. Mijatovic T, Mahieu T, Bruyère C et al. UNBS5162, a novel naphthalimide that decreases CXCL chemokine expression in experimental prostate cancers. Neoplasia. 2008 Jun;10(6):573-86.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 326.35 |
Cas No. | 956590-23-1 |
Formula | C17H18N4O3 |
Solubility | Soluble in DMSO |
Chemical Name | 1-(2-(2-(dimethylamino)ethyl)-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinolin-5-yl)urea |
SDF | Download SDF |
Canonical SMILES | CN(C)CCN1C(C2=C3C(C=CC=C3C1=O)=CC(NC(N)=O)=C2)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |