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UM 171

现货
Catalog No.
A8950
造血干细胞激动剂。
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 1,500.00
现货
5mg
¥ 1,050.00
现货
25mg
¥ 3,400.00
现货

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Background

UM171 is a selective agonist of human hematopoietic stem cell self-renewal [1].

UM171 is a synthesized analog of UM729, which is screened out for its ability to expand human CD34+ CD45RA– mobilized peripheral blood cells. UM171 was 10- to 20- fold more potent than UM729 with efficacious concentrations of 17 to 19 nM. UM171 treatment enhanced the engraftment potential of CD34+ macaque cells by threefold. It was also found that UM171 had no direct effect on mitosis and no effect on the division rate of phenotypically primitive populations. In addition, UM171 showed cooperation with StemRegenin (SR1) to enhance expansion of short-lived progenitors while UM171 itself selectively enhanced the long-term-HSCs. Moreover, in NSG mice, UM171 exerted effects on lymphoid-deficient differentiation in human hematopoietic reconstitution obtained by transplanting fresh or expanded cells [1].

References:
[1] Fares I, Chagraoui J, Gareau Y, et al. Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal. Science, 2014, 345(6203): 1509-1512.

文献引用

1. Kevin A. Goncalves, Megan D. Hoban, et al. "Comparison of hematopoietic stem cell expansion methods reveals that aryl hydrocarbon receptor inhibition optimally expands CD90+ engrafting cells." Cell Stem Cell.2 Apr 2018

Chemical Properties

StorageStore at -20°C
M.Wt453.54
Cas No.1448724-09-1
FormulaC25H27N9
Solubility≥22.7mg/mL in DMSO
Chemical Name(1r,4r)-N1-(2-benzyl-7-(2-methyl-2H-tetrazol-5-yl)-9H-pyrimido[4,5-b]indol-4-yl)cyclohexane-1,4-diamine
SDFDownload SDF
Canonical SMILESN[C@@H](CC1)CC[C@H]1NC2=NC(CC3=CC=CC=C3)=NC(N4)=C2C5=C4C=C(C6=NN(C)N=N6)C=C5
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验[1]:

细胞系

CD34+ 型CB细胞

溶解方法

溶解于DMSO。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。

反应时间

35 nM,3、12、24、48和72小时

应用

用35 nM的UM171处理3、12、24、48和72小时,在每个时间点裂解细胞,提取RNA并测序。UM171显著抑制与红系和巨核细胞分化相关的转录物。在UM171处理的细胞中,只有6-7个基因是普遍上调或下调的,上调幅度最大的基因编码表面分子。这些基因包括PROCR(也称为EPCR或CD201),是小鼠LT-HSCs的标志物。

动物实验[1]:

动物模型

雌性NSG小鼠

剂量

35 nM UM171处理的CD34+ CB细胞

应用

将UM171或DMSO处理的CD34+ CB细胞注射到NSG小鼠中,在约300只小鼠中评估人细胞的植入水平,并以热图的形式表示。数据分析表明人类重组的两个新兴模式,一个来自主要的lymphomyeloid LT-HSCs,在高细胞剂量时出现,另一个来自LT-HSCs,具有淋巴缺陷分化的表型,主要在UM171处理中观察到。B淋巴细胞的增殖与lymphomyeloid LT-HSCs的频率或数量均不受UM171影响。UM171对LT-HSC的影响在移植后30周仍然存在,在高细胞剂量时其多向贡献明显。

注意事项

请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。

References:

[1] Fares I, Chagraoui J, Gareau Y, et al. Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal. Science, 2014, 345(6203): 1509-1512.

质量控制

化学结构

UM 171

相关生物数据

UM 171