UF 010
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
UF 010是一种新型的I类HDAC选择性抑制剂,对HDAC1\HDAC2\HDAC3\HDAC8\HDAC6\HDAC10和HDAC11的IC50值分别为0.5 μM\0.1 μM\0.06 μM\1.5 μM\9.1 μM\15.3 μM和44.5 μM[1].
组蛋白去乙酰化酶(HDACs)从组蛋白的ε-N-乙酰赖氨酸上移除乙酰基,使组蛋白更紧密地包裹DNA.DNA表达由去乙酰化和乙酰化调节.
UF 010是一种新型的I类HDAC选择性抑制剂,是一种快速起效但缓慢关闭的抑制剂.在荧光实验中,UF 010(5 nM ~ 100 μM)具有显著抑制作用,对HDAC1\HDAC2\HDAC3\HDAC8\HDAC6\HDAC10和HDAC11的IC50值分别为0.5 μM\0.1 μM\0.06 μM\1.5 μM\9.1 μM\15.3 μM和44.5 μM,对HDAC4\HDAC5\HDAC7和HDAC9的IC50值均大于100 μM.在HCT116细胞中,UF 010促进乙酰化组蛋白在所有检测位点的积累,并显著地抑制细胞HDAC(其IC50值为1.8 μM).此外,UF 010还能杀死细胞,其IC50值为11.2 μM.在肝癌细胞系HepG2中,UF 010抑制细胞存活,其IC50值为4.6 μM.在MDA-MB-231细胞中,UF 010呈剂量依赖性地使细胞阻滞于G1/S期,增加G1期细胞以及减少S期细胞.1 μM UF010显著地减慢细胞迁移[1].
参考文献：
[1]. Wang Y, Stowe RL, Pinello CE, et al. Identification of histone deacetylase inhibitors with benzoylhydrazide scaffold that selectively inhibit class I histone deacetylases. Chem Biol, 2015, 22(2): 273-284.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 271.15 |
| Cas No. | 537672-41-6 |
| Formula | C11H15BrN2O |
| Solubility | insoluble in H2O; ≥10.65 mg/mL in DMSO; ≥20.85 mg/mL in EtOH |
| Chemical Name | 4-bromo-N'-butylbenzohydrazide |
| SDF | Download SDF |
| Canonical SMILES | O=C(NNCCCC)C1=CC=C(Br)C=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
