ubiquitin specific protease 3 fragment
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
泛素特异性蛋白酶3（USP3）是uH2A和uH2B的去泛素化酶。体内USP3与染色质动态关联，去泛素化H2A / H2B。USP3的ZnF-UBP结构域介导uH2A-USP3相互作用。通过RNAi消除USP3的功能，导致S期延迟和DNA断裂的积累，随后激活DNA损伤检查点通路。响应于电离辐射，在g-H2AX DNA修复病灶中，uH2A重新分布和共定位（1），USP3对于uH2A/泛素结合物的去泛素化和g-H2AX去磷酸化具有重要作用（2）。USP3是H2A和H2B泛素化的新型调节剂，阐明了其在防止复制压力方面的作用，参与对DNA双链断裂的应答。
USP3已被表征为体外功能性的DUB，人DUB与酿酒酵母（S. cerevisiae）Ubp8最同源，参与调节H2B去泛素化4。
Figure1. Formula of Ubiquitin specific protease 3 fragment
参考文献：
1.F. Nicassio, N. Corrado et al. Human USP3 Is a Chromatin Modifier Required for S Phase Progression and Genome Stability. Current Biology 17, 1972–1977.
2.Sloper-Mould, K.E., Eyre, H.J., Wang, X.W., Sutherland, G.R., and Baker, R.T. (1999). Characterization and chromosomal localization of USP3, a novel human ubiquitin-specific protease. J. Biol. Chem. 274, 26878–26884.
3.Henry, K.W., Wyce, A., Lo, W.S., Duggan, L.J., Emre, N.C., Kao, C.F., Pillus, L., Shilatifard, A., Osley, M.A., and Berger, S.L. (2003). Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8. Genes Dev. 17, 2648–2663.
4.Zhang, Y. (2003). Transcriptional regulation by histone ubiquitination and deubiquitination. Genes Dev. 17, 2733–2740.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 937.07 |
| Formula | C38H68N10O15S |
| Synonyms | H2N-Ser-Thr-Thr-Ala-Ile-Cys-Ala-Thr-Gly-Leu-OH |
| Solubility | ≥93.7 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
| SDF | Download SDF |
| Canonical SMILES | NC(CO)C(NC(C(O)C)C(NC(C(C)O)C(NC(C)C(NC(C(C)CC)C(NC(CS)C(NC(C)C(NC(C(O)C)C(NCC(NC(CC(C)C)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
