TRC 051384
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
靶点：Hsp70
IC50: N/A
TRC 051384属于取代的2-丙烯-1-酮类，是热休克蛋白70（HSP70）的有效诱导剂[1]。HSP70的诱导是应激细胞的自然反应，保护神经细胞免受不同的损伤，包括急性缺血。因此，HSP70的诱导提供了中风潜在的治疗靶标[1]。
体外实验：在HeLa细胞中，6.25和12.5 μM的TRC 051384诱导的HSP70参与热休克因子-1（HSF1）的激活，并引起分子伴侣和抗炎活性的增加[1]。此外，10-50 μM的TRC 051384剂量依赖性地诱导内皮细胞以及白细胞中HSPA1B的过表达[2]。
体内实验：在短暂性脑缺血中风大鼠模型中，腹腔给药9 mg/kg的TRC 051384显著降低中风相关的神经元损伤、残疾，提高存活率[1]。此外，在野生型小鼠中，腹腔给药9 mg/kg的TRC 051384延迟血栓形成，且没有增加出血风险[2]。
参考文献：
1. Mohanan A, Deshpande S, Jamadarkhana PG, Kumar P, Gupta RC, Chauthaiwale V, et al. Delayed intervention in experimental stroke with TRC 051384--a small molecule HSP70 inducer. Neuropharmacology. 2011;60(6):991-9.
2. Allende M, Molina E, Guruceaga E, Tamayo I, Gonzalez-Porras JR, Gonzalez-Lopez TJ, et al. Hsp70 protects from stroke in atrial fibrillation patients by preventing thrombosis without increased bleeding risk. Cardiovasc Res. 2016;110(3):309-18.
| Physical Appearance | Yellow solid |
| Storage | Store at -20°C |
| M.Wt | 465.54 |
| Cas No. | 867164-40-7 |
| Formula | C25H31N5O4 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥109 mg/mL in DMSO |
| Chemical Name | (Z)-N'-(2-morpholinoethyl)-N-(4-((E)-3-(6-morpholinopyridin-2-yl)acryloyl)phenyl)carbamimidic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(C1=CC=C(N/C(O)=N/CCN2CCOCC2)C=C1)/C([H])=C([H])/C3=NC(N4CCOCC4)=CC=C3 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
