Tolbutamide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
甲苯磺丁脲(Tolbutamide)是一种有效的cAMP抑制剂,IC50值为4 mM[1].
已报道甲苯磺丁脲对基础和环磷酸腺苷激活的蛋白激酶活性都有抑制作用,对cAMP依赖性蛋白激酶的IC50值为4 mM.此外,甲苯磺丁脲已被发现可抑制来自犬心脏的可溶性及膜结合蛋白激酶.而且,甲苯磺丁脲抑制脂肪组织的环磷酸腺苷依赖性蛋白激酶,这正是其抗脂解作用的原因[1].此外,甲苯磺丁脲和dbcAMP可增加约四倍Cx43 mRNA的水平,以及减少约80%的Ki-67表达[2].
参考文献：
[1] Wray HL, Harris AW. Adenosine 3', 5'-monophosphate-dependent protein kinase in adipose tissue: inhibition by tolbutamide. Biochem Biophys Res Commun. 1973 Jul 2;53(1):291-4.
[2] Sánchez-Alvarez R1, Paíno T, Herrero-González S, Medina JM, Tabernero A. Tolbutamide reduces glioma cell proliferation by increasing connexin43, which promotes the up-regulation of p21 and p27 and subsequent changes in retinoblastoma phosphorylation. Glia. 2006 Aug 1;54(2):125-34.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 270.35 |
| Cas No. | 64-77-7 |
| Formula | C12H18N2O3S |
| Solubility | insoluble in H2O; ≥10.05 mg/mL in DMSO; ≥15.83 mg/mL in EtOH |
| Chemical Name | 1-butyl-3-(4-methylphenyl)sulfonylurea |
| SDF | Download SDF |
| Canonical SMILES | CCCCNC(=O)NS(=O)(=O)C1=CC=C(C=C1)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
