Tigecycline mesylate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Storage | Store at -20°C |
M.Wt | 681.75 |
Cas No. | 1135871-27-0 |
Formula | C30H43N5O11S |
Solubility | Soluble in DMSO |
Chemical Name | (4S,4aS,5aR,12aS)-9-((Z)-(2-(tert-butylamino)-1-hydroxyethylidene)amino)-4,7-bis(dimethylamino)-3,10,12,12a-tetrahydroxy-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carbimidic acid compound with methanesulfonic acid (1:1) |
SDF | Download SDF |
Canonical SMILES | CC(C)(NC/C(O)=N/C1=CC(N(C)C)=C(C2=C1O)C[C@@](C(C2=O)=C(O)[C@@]34O)([H])C[C@@]3([H])[C@@](N(C)C)([H])C(O)=C(C(O)=N)C4=O)C.CS(O)(=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
人白血病细胞 |
溶解方法 |
在DMSO中是可溶的。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
0.63~30 µM |
应用 |
研究结果表明,tigecycline由于具有抑制线粒体翻译的能力,因此它还具有针对急性髓性白血病(AML)的体外和体内活性。Tigecycline在重建后相对不稳定,这种不稳定性可能会限制在非卧床输注中使用该药物来治疗感染,并可能阻止制定最佳剂量方案来治疗AML。 |
动物实验 [1]: | |
动物模型 |
急性骨髓性白血病(AML)异种移植的NOD / SCID小鼠模型 |
剂量 |
50mg/kg(i.p.) ,每天两次,持续11天 |
应用 |
Tigecycline和新型制剂均以相似的功效降低了肿瘤的体积和重量。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同,这是由实验系统的误差引起的,属于正常现象。 |
References: [1]. Jitkova Y, Gronda M, Hurren R, Wang X, Goard CA, Jhas B, Schimmer AD. A novel formulation of tigecycline has enhanced stability and sustained antibacterial and antileukemic activity. PLoS One. 2014 May 28;9(5):e95281. doi: 10.1371/journal.pone.0095281. eCollection 2014. PubMed PMID: 24871339; PubMed Central PMCID: PMC4037176. |