Tetracycline Hydrochloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Tetracycline Hydrochloride is an inhibitor of bacterial ribosomes [1].
Tetracycline Hydrochloride is believed to inhibit translation by binding to the 16S rRNA and inhibiting the binding of aminoacyl-tRNA to the mRNA-ribosome complex. However, the exact target site(s) and mechanism(s) of action remain subjects of much debate [1].
Tetracycline Hydrochloride at doses of 0, 10, 25, 50, 75, 150, 225 and 300 µM exhibited increasing antimicrobial effect against resistant strains of Staphylococcus aureus (S. aureus). The statistically evaluated IC50 values of Tetracycline Hydrochloride against S. aureus, cadmium-resistant S. aureus, and lead-resistant S. aureusafter first 6 h were 4.8, 3.8, 2.2 µM, respectively [2].
In humans who had minimum Propionibacterium acnes (P. acnes) levels of 10000 colony-forming units per cm2 at baseline, Tetracycline Hydrochloride treatment (1000 mg/d, b.i.d., p.o.) over a 6-week period significantly reduced P. acnes on both cheeks and forehead after 3 and 6 weeks, but produced little to no residual effect. At 9 weeks, P. acnes levels in the Tetracycline Hydrochloride group had returned to baseline [3].
References:
[1]. Chukwudi C U. rRNA Binding Sites and the Molecular Mechanism of Action of the Tetracyclines. Antimicrobial Agents and Chemotherapy, 2016, 60(8): 4433-4441.
[2]. Chudobova D, Dostalova S, Blazkova I, et al. Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus. International Journal of Environmental Research and Public Health, 2014, 11(3): 3233-3255.
[3]. Leyden J J, Kaidbey K, Gans E H. The antimicrobial effects in vivo of minocycline, doxycycline and tetracycline in humans. Journal of Dermatological Treatment, 1996, 7: 223-225.
Storage | Store at -20°C |
M.Wt | 480.90 |
Cas No. | 64-75-5 |
Formula | C22H25ClN2O8 |
Solubility | insoluble in EtOH; ≥12.02 mg/mL in DMSO with gentle warming; ≥57.7 mg/mL in H2O |
Chemical Name | (4S,4aS,5aS,6S,12aR)-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide;hydrochloride |
SDF | Download SDF |
Canonical SMILES | CC1(C2CC3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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