TCID
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
TCID是有效的、选择性的和细胞渗透性的UCHL3(泛素羧基末端酯酶L3(泛素硫酯酶))抑制剂,其IC50值为0.6 μM。TCID也是UCH-L1的抑制剂,但对其抑制活性比对UCHL3弱125倍,其IC50值为75 μM[1]。
UCHL3属于去泛素化酶家族,移除多肽上的泛素。UCHL3加工泛素前体和泛素化蛋白以调节细胞泛素化水平。
在脑干和脊髓原代神经元中,TCID(10 μM)对UCHL3的抑制作用以及LDN-57444(10 μM)对UCHL1的抑制作用可以减弱GlyT2泛素化,在经抑制剂长期处理的细胞中,此效果尤其显著[2]。在转染的MDCK细胞中,TCID(10 μM,30 mins)不影响UCH-L1抑制剂对细胞囊泡中YFP-GLT-1积累的促进作用[3]。
参考文献:
[1] Liu Y, Lashuel HA, Choi S, Xing X, Case A, Ni J, Yeh LA, Cuny GD, Stein RL, Lansbury PT Jr. Discovery of inhibitors that elucidate the role of UCH-L1 activity in the H1299 lung cancer cell line. Chem Biol. 2003 Sep;10(9):837-46.
[2] de Juan-Sanz J, Núez E, López-Corcuera B, Aragón C. Constitutive endocytosis and turnover of the neuronal glycine transporter GlyT2 is dependent on ubiquitination of a C-terminal lysine cluster. PLoS One. 2013;8(3):e58863.
[3] Martínez-Villarreal J, García Tardón N, Ibáez I, Giménez C, Zafra F. Cell surface turnover of the glutamate transporter GLT-1 is mediated by ubiquitination/deubiquitination. Glia. 2012 Sep;60(9):1356-65.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 283.92 |
Cas No. | 30675-13-9 |
Formula | C9H2Cl4O2 |
Solubility | insoluble in EtOH; insoluble in H2O; ≥16.45 mg/mL in DMSO |
Chemical Name | 4,5,6,7-tetrachloroindene-1,3-dione |
SDF | Download SDF |
Canonical SMILES | C1C(=O)C2=C(C1=O)C(=C(C(=C2Cl)Cl)Cl)Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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