TCEP hydrochloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
三(2-羰基乙基)磷盐酸盐(TCEP HCL) 是一种水溶性的还原剂,可以断开二硫键。它是一种非硫醇类和非挥发性的固体。可用作还原磺酰氯化物,氮氧化合物,二甲亚砜和叠氮化物的催化剂。
在6周大小鼠的组织和血浆中测量脱氧化坏血酸(DHA)时,溶解在pH 1.5的5%偏磷酸含1 mM EDTA的35 mM TCEP HCL,在冰上放置两个小时后,能完全把DHA还原成抗坏血酸(AA)[1]。在氏曲霉蛋白酶XIII型和猪的胃蛋白酶中,TCEP HCL 也可以用于蛋白酶解和氢/氘交换 [2]。
参考文献:
1. Sato Y, Uchiki T, Iwama M, Kishimoto Y et al. Determination of dehydroascorbic acid in mouse tissues and plasma by using tris(2-carboxyethyl)phosphine hydrochloride as reductant in metaphosphoric acid/ethylenediaminetetraacetic acid solution. Biol Pharm Bull. 2010;33(3):364-9.
2. Zhang HM, McLoughlin SM, Frausto SD et al. Simultaneous reduction and digestion of proteins with disulfide bonds for hydrogen/deuterium exchange monitored by mass spectrometry. Anal Chem. 2010 Feb 15;82(4):1450-4.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 286.65 |
Cas No. | 51805-45-9 |
Formula | C9H16ClO6P |
Solubility | ≥28.7 mg/mL in H2O; insoluble in EtOH; ≥25.7 mg/mL in DMSO |
Chemical Name | 3,3',3''-phosphinetriyltripropanoic acid hydrochloride |
SDF | Download SDF |
Canonical SMILES | O=C(O)CCP(CCC(O)=O)CCC(O)=O.Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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